Cyclin; DNA polymerase delta auxiliary protein; PCNA;
Application:
WB ICC/IF IP
Reactivity:
Human,Mouse,Monkey,Rat,Hamster
Dilution:
WB 1:1000 ICC/IF 1:100 IP
Western blot detection of PCNA in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.
Immunofluorescent analysis of HeLa cells using PCNA mouse mAb (dilution 1:100).Fixed in 100% methanol for 2hr at -20℃.
Immunoprecipitation analysis of Hela cell lysates using PCNA mouse mAb.
Purification:
Affinity-chromatography
Specificity:
PCNA Mouse mAb detects endogenous levels of total PCNA
Immunogen:
Purified recombinant human PCNA protein fragments expressed in E.coli.
Description:
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.