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- 详细信息
- 文献和实验
- 技术资料
- CAS号:
见产品说明书
- 英文名:
Yeast Extract Glucose Chloramphenicol Agar
- 保质期:
见产品说明书
- 保存条件:
见产品说明书
- 库存:
大量
- 规格:
500 g
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文献和实验Yeast Nuclear Extract (Small Scale)
Yeast Nuclear Extract (Small Scale) Hahn Lab (adapted from J.Leatherwood,Ptashne lab) last modified Mon,Aug 27,2001 Cell Growth 3 liters of cells are grown in YPD (3% glucose)to A600 of 3-5.Antifoam A (two drops from a P200 pipetman)is added
Yeast Nuclear Extract (Small Scale)
(volumes given are for 6 2l extracts) 50 mM Tris,7.5 250 ml 1.5g Tris in 250 ml H2O pH to 7.5 with HCl Before use,add 4.6 mg DTT/ml YPD/S 2.5 liters at room temp .(YPD with 1M sorbitol) 25g Yeast extract 50g Bactopeptone 50g Dextrose (glucose) 455g
Pol III whole cell yeast extract (small scale)
Pol III whole cell yeast extract (small scale) Mike Schultz, Pavel Aprikian, Steve Hahn last modified6/28/99 Grow 2 liters yeast in YPD (with 3% glucose) to an A600 of 1.5 to 2.0 (inoculate ~1.5 ml saturated overnight
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