- $265
- Ybscience
- Yb--3195
- 美国/德国/中国
- 2026年05月21日
- WB ICC/IF
- Mouse
- 详见说明书
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
SAFB1 Mouse mAb
- 抗体英文名：
SAFB1 Mouse mAb
- 靶点：
详见说明书
- 浓度：
1.0mg/ml
- 应用范围：
WB ICC/IF
- 宿主：
Mouse
- 供应商：
上海钰博生物科技有限公司
- 库存：
大量
- 目录编号：
3195
- 抗原来源：
Mouse
- 保质期：
1年
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20°C
- 形态：
液体或冻干粉
- 亚型：
详见说明书
- 免疫原：
详见说明书
- 规格：
50μg/50μl, 100μg/100μl
-
- Catalog No. : Yb--3195
- Size : 50μg/50μl, 100μg/100μl
-
- Mol Weight : 130KDa
- UniProt : Q15424
-
- Isotype : Mouse IgG1
- Clonality : Monoclonal
-
- Price : $265/100μg
- Availability : 3-4 days
Category
- Primary Antibodies
- Secondary antibody
- Loading control / tag antibody
Details
Alternative Names:
HAP; HET;HSP27 ERE TATA binding protein; HSP27 ERE-TATA-binding protein; SAF-B1; SAFB1; scaffold attachment factor B1;
Application:
WB ICC/IF
Reactivity:
Human
Dilution:
WB 1:1000 ICC/IF 1:200
 |
Western blot detection of SAFB1 in HelaNE,A431,MDA-MB-468,Breast cancer,ZR75-1 and MCF7 cell lysates using SAFB1 mouse mAb (1:4000 diluted).Predicted band size: 130kDa.Observed band size: 130kDa. |
 |
Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using SAFB1 mouse mAb (dilution 1:200). |
Purification:
Affinity-chromatography
Specificity:
SAFB1 Mouse mAb detects endogenous levels of total SAFB1
Immunogen:
Purified recombinant human SAFB1 protein fragments expressed in E.coli.
Description:
Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing By similarity. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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