6*His; His Tag; 6histag; 6XHis; HHHHHH; HISTIDINE TAGGED; HRP 6XHis; HRP HHHHHH; HRP His tag;
Application:
WB
Reactivity:
All
Dilution:
WB 1:1000~1:5000
Western blot analysis of His recombinant protein with His Mouse mAb HRP conjugated diluted at (1) 1:1000; (2) 1:5000.
Purification:
Affinity-chromatography
Specificity:
His-Tag Antibody(HRP conjugated) detects endogenous levels of His-tagged proteins.
Immunogen:
A synthetic peptide HHHHHH coupled to KLH
Description:
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibody have been widely used in study of protein expression in various systems. Our anti-His tag monoclonal antibody provides a simple solution to detect the expression of protein with a His tag.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -23°C long term. Avoid freeze / thaw cycle.