- ¥4290
- 进口
- ELR-Insulin-1
- 美国
- 2025年12月10日
11 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 检测方法：
ELISA
- 库存：
2
- 供应商：
上海研卉生物科技有限公司
- 规格：
96T
大鼠胰岛素ELISA Kit rat Insulin ELISA kit
1 Plate Kit (3900)
2 Plate Kit (7020)
5 Plate Kit (15600）
1 Plate Kit (3900)
2 Plate Kit (7020)
5 Plate Kit (15600）
Sensitivity
5 µIU/mL
Detection Range
5 µIU/ml - 300 µIU/mL
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Specificity
This ELISA antibody pair detects rat, mouse, human, porcine and bovine Insulin and Proinsulin.
Species Detected
Rat
Protein Name & Synonyms
Insulin-1
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
大鼠胰岛素Insulin ELISA Kit
¥4290
