人c-jun ELISA Kit

c-Jun (Total) InstantOne™ ELISA

Also known as: JUN, Transcription factor AP-1

RUO: For Research Use Only. Not for use in diagnostic procedures.

Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of total human and mouse c-Jun in cell lysates. Detection of rat c-Jun is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Jun is a member of the Activator Protein-1 (AP-1) family of transcription factors. AP-1 proteins can form homodimers (e.g., Jun/Jun) or heterodimers with other family members (e.g., Jun/Fos). AP-1 activity is highly regulated in normal cells by phosphorylation in response to growth factors, cytokines, and various other intra- and extracellular signals. Jun is phosphorylated at Ser63 and Ser73 by JNK, resulting in the ability of Jun/Jun or Jun/Fos dimers to bind DNA and modulate transcription.

Reactivity	Human
Suitable Sample Types	Tested on cell culture samples. Expected to work on many other sample types, but have not been tested at this time.
Components	The kit includes sufficient reagents to perform 96 wells of the kit specific target. User Manual InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed) Cell Lysis Buffer (5X) (Part No. IOCLB1) - 8 mL Enhancer Solution (Part No. IOES1) - 0.5 mL Wash Buffer (10X) (Part No. IOWB1) - 8 mL c-Jun (Total) Capture Antibody Reagent (Part No. IOJUNCAR1) - 3 mL c-Jun (Total) Detection Antibody Reagent (Part No. IOJUNDAR1) - 3 mL Positive Control Cell Lysate (lyophilized) (Part No. IOJUNPC1) - 1 ea Detection Reagent (TMB Substrate) (Part No. IODR1) - 10 mL Stop Solution (Part No. IOSS1) - 10 mL Plate Seal - 1 ea
Reported Applications	ELISA

Cat. No.	Name	Excite	Emit	Application	Reg.
IOCLB	InstantOne ELISA Cell Lysis Buffer (5X)			ELISA	RUO
85-86162	phospho-c-Jun (Ser73) InstantOne™ ELISA			ELISA	RUO
IOWB	InstantOne ELISA Wash Buffer(10X)			ELISA	RUO
85-86163	c-Jun (Total/Phospho) InstantOne™ ELISA			ELISA	RUO