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- PEPROTECH
- 500-P84BT
- 500-P84BT
- 2025年07月15日
- WB ELISA
- 兔
- 人大小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
500-P84BT
- 靶点：
Biotinylated Anti-Human/Murine/Rat BDNF-生物素标记
- 级别：
科研
- 目录编号：
500-P84BT
- 克隆性：
多克隆
- 抗原来源：
Recombinant Human/Murine/Rat BDNF (PeproTech catalog# 450-02)
- 抗体英文名：
Biotinylated Anti-Human/Murine/Rat BDNF-生物素标记
- 抗体名：
Biotinylated Anti-Human/Murine/Rat BDNF-生物素标记
- 标记物：
生物素标记
- 宿主：
兔
- 适应物种：
人大小鼠
- 应用范围：
WB ELISA
- 浓度：
Biotinylated Anti-Human/Murine/Rat BDNF-生物素标记
- 规格：
25ug
Source: Polyclonal Rabbit
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BDNF. Anti-Human/Murine/Rat BDNF-specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen: E.coli derived Recombinant Human/Murine/Rat BDNF (PeproTech catalog# 450-02)
Sandwich ELISA: To detect hBDNF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human BDNF (500-P84) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBDNF.
Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
| 500-P84-50 | Rabbit Anti-Human BDNF | 50ug |
| 500-P84-100 | Rabbit Anti-Human BDNF | 100ug |
| 500-P84-1000 | Rabbit Anti-Human BDNF | 1000ug |
| 500-P84Bt-25 | Biotinylated Rabbit Anti-Human BDNF | 25ug |
| 500-P84Bt-50 | Biotinylated Rabbit Anti-Human BDNF | 50ug |
| 500-P84Bt-1000 | Biotinylated Rabbit Anti-Human BDNF | 1000ug |
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
cold wash buffer (PBS/0.1% NaN3/1.0% fetal bovine serum). Centrifuge at 350 x g for 5 min. Finally, resuspend cell pellet to a concentration of 2 x 107 cells/ml (i.e., 106 cells per 50 µl). Dilute primary mAbs (e.g., unconjugated, biotinylated
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
are performed by inducing in vitro and in vivo apoptosis and then visualized the phenomenon with anti-DNA mAb. In vitro experiments are performed by treating human lymphocytes with 2-deoxyribose and rat thymocytes with dexamethasone 21-phosphate. In vivo
Cell Surface Immunofluorescence Staining Protocol
16/32 on ice for 10 minutes. In the absence of an effective/available blocking antibody for human and/or rat Fc receptors, an alternative approach is to pre-block cells with excess irrelevant purified Ig from the same species and same isotype
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