LS-F8321 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human CBLN1 / Cerebellin 1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CBLN1 / Cerebellin 1 as low as 0.054 nanograms per millilter.
About CBLN1 / Cerebellin 1
Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. P23435NM_004352NP_004343.1
Cerebellin 1 (CBLN1) No significant cross-reactivity or interference between Cerebellin 1 (CBLN1) and analogs was observed.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.054 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Coated 96-well Strip Plate
Adhesive Plate Sealers
Standard (Lyophilized)
Sample Diluent
Detection Reagent A (Green)
Detection Reagent B (Red)
Assay Diluent A
Assay Diluent B
TMB Substrate
Stop Solution
Wash Buffer (30x concentrate)
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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