LS-F7232 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Mouse CAT / Catalase in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CAT / Catalase as low as 28.9 picograms per milliliter.
About CAT / Catalase
CAT / Catalase is catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. P04040NM_001752NP_001743.1
Catalase (CAT) No significant cross-reactivity or interference between Catalase (CAT) and analogs was observed.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Detection Range
62.5 - 4000 pg/ml
Sensitivity
28.9 pg/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Coated 96-well Strip Plate
Adhesive Plate Sealers
Standard (Lyophilized)
Sample Diluent
Detection Reagent A (Green)
Detection Reagent B (Red)
Assay Diluent A
Assay Diluent B
TMB Substrate
Stop Solution
Wash Buffer (30x concentrate)
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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