LS-F7587 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human CACNA1D / Cav1.3 in samples of Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CACNA1D / Cav1.3 as low as 0.121 nanograms per millilter.
About CACNA1D / Cav1.3
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. Q01668NM_000720NP_000711.1
Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) and analogs was observed.
Manual
Intended Sample Types
Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Detection Range
0.313 - 20 ng/ml
Sensitivity
0.121 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Coated 96-well Strip Plate
Adhesive Plate Sealers
Standard (Lyophilized)
Sample Diluent
Detection Reagent A (Green)
Detection Reagent B (Red)
Assay Diluent A
Assay Diluent B
TMB Substrate
Stop Solution
Wash Buffer (30x concentrate)
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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