Blank control:HepG2.
Primary Antibody (green line): Mouse Anti- CD90 antibody (bsm-30105M)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Mouse IgG .
Secondary Antibody : Goat anti-Mouse IgG-CY5
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1880
- Bioss
- bsm-30105M
- 2025年10月16日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ug
| 产品编号 | bsm-30105M |
| 英文名称 | human CD90/Thy-1 Mouse mAb |
| 中文名称 | 小鼠抗人CD90单克隆抗体 |
| 英文别名 | CD90/Thy1; CD7; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN. |
| 中文别名 | CD7; CD90 antigen; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; |
| 产品应用 | Flow-Cyt=1:50-100 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 免疫原 | HEL cells |
| 亚型 | Mouse IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 12 kDa |
| 实际分子量 | 25-35kDa |
| 浓度 | 0.5mg/ml |
| 储存液 | 0.01M PBS (pH7.4). |
| 研究领域 | Immunology > Adaptive Immunity > T Cells > CD Neuroscience > Cell Type Marker > Neuron marker > Synapse marker Stem Cells > Hematopoietic Progenitors > Lymphoid > B Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Monocytic Lineage |
| 亚基 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 组织特异性 | Abundant in lymphoid tissues. |
| 翻译后修饰 | Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus. |
| 相似性 | Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1:50-100} |
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human CD90/Thy-1 Mouse mAb(bsm-30105M)-100ug
￥1880
