文献支持

Caspase-9 Rabbit pAb(bs-20773R

)-50ul/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-20773R
更新日期2025年10月24日

应用范围产品信息以Bioss网站为准

北京博奥森生物技术有限公司

品牌商

10 年钻石会员

相关产品推荐更多 >

万千商家帮你免费找货

0 人在求购买到急需产品

详细信息
文献和实验
技术资料

应用范围：
产品信息以Bioss网站为准
规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-20773R
英文名称	Caspase-9 Rabbit pAb
中文名称	活化半胱胺酸蛋白酶蛋白-9抗体
英文别名	Caspase-9 subunit p35; Apaf-3; APAF 3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease MCH 6; Apoptotic protease MCH6; CASP 9; CASP9; Caspase 9; Caspase 9 apoptosis related cysteine protease; Caspase 9 precursor; Caspase 9c; Caspase9; Caspase9 subunit p35; ICE LAP6; ICE like apoptotic protease 6; RNCASP9; MCH 6; MCH6; OTTHUMP00000044594; CASP9_HUMAN.
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human, Mouse, Rat (Pig, Cow, Rabbit, Sheep)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human Caspase-9
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	35/50 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cancer > Cell Death > Apoptosis > Apoptosis Markers > Caspases Cancer > Cell Death > Apoptosis > Apoptosis Markers > Cytochrome C Cancer > Cell Death > Apoptosis > Metabolism Cancer > Invasion/microenvironment > Apoptosis > Caspases Cancer > Invasion/microenvironment > Apoptosis > Cytochrome C Cell Biology > Apoptosis > Intracellular > Caspases etc > Caspases Metabolism > Pathways and Processes > Metabolism processes > Apoptosis
亚基	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts (inactive form) with EFHD2. Interacts with HAX1. Interacts with BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce and BIRC7/livin.
组织特异性	Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
翻译后修饰	Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation.
相似性	Belongs to the peptidase C14A family. Contains 1 CARD domain.
功能	Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{ICC/IF}	{1:100-500}
{IF}	{1:100-500}
{Flow-Cyt}	{1ug/Test}

P‌‌araformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (bs-20773R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

P‌‌araformaldehyde-fixed, paraffin embedded (Rat urinary bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (bs-20773R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-9 (bs-20773R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35/50 kD
Observed band size: 35 kD

Sample:
Urinary bladder(Mouse) Lysate at 40 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase-9 (bs-20773R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46-51'35'37 kD
Observed band size: 35 kD

P‌‌araformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (bs-20773R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

P‌‌araformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-9) Polyclonal Antibody, Unconjugated (bs-20773R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

HepG2 cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-9) polyclonal Antibody, Unconjugated (bs-20773R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Sample: K562 Cell (Human) Lysate at 40 ug
Primary: Anti-Caspase-9 (bs-20773R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35/50 kD
Observed band size: 35 kD

Overlay histogram showing Hela cells(1ug/1x10^6 cells) stained with bs-20773R (Green line). The cells were fixed with 80% methanol (5 min) and and then permeabilized with 0.01M PBS-Tween for 20 min . The cells were then incubated in 1x PBS/10% normal goat serum to block non-specific protein-protein interactions followed by the antibody for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs-0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

文献和实验

该产品被引用文献

[IF={{ 9.8 }}] {Bohan Chen. et al. Inhalation of ammonia promotes apoptosis and induces autophagy in hepatocytes via Bax/BCl-2 and m-TOR/ATG5/LC-3bII axes. SCI TOTAL ENVIRON. 2024 Feb;912:169036} {WB} {Mouse}

[IF={{ 7.169 }}] {Zhu Sai-ya. et al. COX17 restricts renal fibrosis development by maintaining mitochondrial copper homeostasis and restoring complex IV activity. ACTA PHARMACOL SIN. 2023 May;:1-12} {WB} {Human，Rat}

[IF={{ 7.129 }}] {Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186} {WB} {Rat, Human}

[IF={{ 4.8 }}] {Danyan Li. et al.Mechanism of Yinxu Weitong Capsule in the treatment of precancerous lesions of gastric cancer based on network pharmacology and experimental validation.journal of ethnopharmacology.2025 Feb 11:341:119303.} {Western blot} {Rat}

[IF={{ 4.2 }}] {Qiong Ma. et al. A Preliminary Study on Quantitative Analysis of Collagen and Apoptosis Related Protein on 1064 nm Laser-Induced Skin Injury. BIOLOGY-BASEL. 2024 Apr;13(4):217} {IHC} {Pig}

大家都在搜

大家都在搜

Caspase-9 Rabbit pAb(bs-20773R

相关产品推荐更多 >

CD14 Rabbit pAb(bs-20796R)-50ul/100ul/200ul

CD62p Rabbit pAb, PE conjugated(bs-10770R-PE)-100ul

CAMKK2 Rabbit pAb, Cy5 conjugated(bs-6253R-Cy5)-100ul

DGAT2 Rabbit pAb(bs-22551R)-50ul/100ul/200ul

GADD45A Rabbit pAb, APC-Cy7 conjugated(bs-1360R-APC-Cy7)-100ul

万千商家帮你免费找货

0 人在求购买到急需产品

关于丁香通

公司信息

个人用户

企业机构

大家都在搜

大家都在搜

Caspase-9 Rabbit pAb(bs-20773R

相关产品推荐更多 >

CD14 Rabbit pAb(bs-20796R)-50ul/100ul/200ul

CD62p Rabbit pAb, PE conjugated(bs-10770R-PE)-100ul

CAMKK2 Rabbit pAb, Cy5 conjugated(bs-6253R-Cy5)-100ul

DGAT2 Rabbit pAb(bs-22551R)-50ul/100ul/200ul

GADD45A Rabbit pAb, APC-Cy7 conjugated(bs-1360R-APC-Cy7)-100ul

万千商家帮你免费找货

0 人在求购买到急需产品

同类产品报价

关于丁香通

公司信息

个人用户

企业机构