Jurkat cells (T-cell leukemia,human) treated with 10 μM of camptothecin for 4 hours(panel right) or untreated control(panel left).
1. Wash cells twice with cold PBS and then resuspend cells in 1×Binding Buffer at a concentration of 1×106 cells/ml.
2. Transfer 100 μl of the solution (1×105cells) to a 5 ml culture tube.
3. Add 5 μl of Annexin V-AF488.
4. Add 5 μl PI.
5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark.
6. Add 400 μl of 1×Binding Buffer to each tube.Analyze by flow cytometry within 1 hr.
- ￥680 - 1580
- Bioss
- BA00102
- 2025年10月17日
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20T/50T/100T
| 规格： | 20T | 产品价格： | ￥680.0 |
|---|---|---|---|
| 规格： | 50T | 产品价格： | ￥1080.0 |
| 规格： | 100T | 产品价格： | ￥1580.0 |
| 产品编号 | BA00102 |
| 英文名称 | Annexin V-AF488 Apoptosis Detection Kit |
| 中文名称 | Annexin V-AF488细胞凋亡检测试剂盒 |
| 英文别名 | Annexin V-AF488/PI Apoptosis Detection Kit; Annexin V-AF488 Apoptosis Detection Kit; Annexin V-AF488/PI Kit; Annexin V-AbBy Fluor® 488/PI Kit; |
| 中文别名 | Annexin V-AF488/PI双染细胞凋亡检测试剂盒; Annexin V-AF488-PI细胞凋亡检测试剂盒; AF488 Annexin V/PI细胞凋亡检测试剂盒; Annexin V-AF488/PI双染细胞凋亡检测试剂盒; ANNEXIN V-AF488/PI凋亡检测试剂盒; 细胞凋亡检测试剂盒; 细胞凋亡试剂盒; |
| 保存条件 | Store at 4℃. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | 磷脂酰丝氨酸（PS）是一种带负电荷的磷脂，正常细胞中，PS只分布在细胞膜脂质双层的内侧，而在细胞凋亡早期，细胞膜 PS由脂膜内侧翻向细胞膜外侧，使PS暴露在细胞膜外表面。Annexin V是一种分子量为35~36kD 的Ca2+依赖性磷脂结合蛋白，与磷脂酰丝氨酸有高度亲和力，故可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。因此Annexin V被公认为检测细胞早期凋亡的灵敏指标之一。将Annexin V进行Alexa Fluor488标记，以标记了的Annexin V作为探针，利用荧光显微镜或流式细胞仪可检测细胞凋亡的发生。碘化丙啶（Propidium Iodide, PI）是一种核酸染料，它不能透过正常细胞或早期凋亡细胞的完整的细胞膜，但对凋亡中晚期的细胞和坏死细胞，PI能够透过细胞膜而使细胞核染红。因此采用Annexin V与PI双染的方法，就可以将处于不同凋亡时期的细胞区分开来。 |
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文献和实验相关实验
protocol - Protocol for Annexin V-FITC Apoptosis Detection Kit (ab14085): 1. Incubation of cells with Annexin V-FITC 1) Induce apoptosis by desired method. 2) Collect 1-5 x 105 cells by centrifugation. 3) Resuspend cells
FACS Procedures for Apoptosis Detection
7-AAD for the PI provided with the kit. Materials: Apoptosis Detection Kit (R&D Systems KNX50) . contains: FITC-conjugated human annexin V, PI, 10x Binding Buffer. 7-Amino-actinomycin (Sigma A-9400)
Apoptosis Measurement by Annexin V Staining
is associated with the condensed chromatin in apoptotic cells as a measure of apoptosis. It may appear as early as 3 h post the apoptosis inducing event and precedes the ability to detect actual DNA fragmentation. The annexin V assay has been widely accepted
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Annexin V-AF488 Apoptosis Detection Kit(BA00102)-20T/50T/100T
￥680 - 1580
