AF™ 488 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date. AF™ 488 (Alexa Fluor® 488 equivalent) is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.
AF™ 488 dye structurally is identical to Invitrogen’sAlexa Fluor® 488
An amine-reactive NHS Ester of AF™ 488 dye is also available atFluoroprobes.com
Alexa Fluor® Dyes is a registered trademark of Invitrogen, part of Thermo Fisher Scientific.