- $750
- LSBio
- 详见官方网站
- LS-F816
- 美国
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Mouse, Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
详见官方网站
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F816 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human C/EBP Beta / CEBPB in samples of Cell Lysates and Nuclear Lysates. It is based upon a DNA Binding assay principle.About C/EBP Beta / CEBPB
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Specifications
TypeDNA-Binding ELISA (enzyme-linked immunosorbent assay) kit
Target
C/EBP Beta / CEBPB
Reactivity
Mouse, Human
Specificity
The CEBPB DNA-Binding ELISA detects endogenous levels of total CEBPB protein.
Manual
Intended Sample Types
Cell Lysates, Nuclear Lysates
Format
12 x 8-Well Microstrips
Detection
Colorimetric - 450nm (TMB)
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- 96-Well dsDNA Oligonucleotide Coated Microplate
- 100x Primary Antibody
- HRP-Conjugated Anti-Rabbit IgG Antibody
- Nuclear Lysate Positive Control
- Wild-Type Consensus dsDNA Oligonucleotide
- Mutant Consensus dsDNA Oligonucleotide
- 10x Wash Buffer
- 2x Binding Buffer
- Primary Antibody Diluent
- Nuclear Wash Buffer
- Cytoplasmic Extraction Buffer
- Nuclear Extraction Buffer
- Ready-to-Use Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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DNA-Binding ELISA Platform Overview
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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
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