Rat IL-1 beta ELISA Kit

Product name:

Rat IL-1 beta ELISA Kit

Tests:

1 X 96 well plate

Sample type:

Serum, Plasma, Cell culture supernatants

Assay type:

Sandwich

Sensitivity:

12.5 pg/mL

Range:

31.25-2000 pg/mL

Reacted Species:

Rat

Tested applications:

Sandwich ELISA

Recovery:

Sample Type	Average	Range
Rat Serum	84%	77%-97%
Cell culture supernatants	107%	93%-118%

IntraAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	20	76.4	3.0	4.0
2	20	243.0	10.2	4.2
3	20	849.2	30.1	4.4

InterAssay:

Sample	n	mean (pg/mL)	SD	CV%
1	24	50.1	4.2	8.3
2	24	190.3	7.3	3.8
3	24	684.7	17.4	2.5

Product overview:

KE20005 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1 beta. The assay recognizes rat IL1-beta. An antibody specific for IL1-beta has been pre-coated onto the microwells. The IL-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1 beta is added to detect the captured IL-1 beta protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Storage Instructions:

All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

Synonyms:

IL 1, IL 1 BETA, IL 1BETA, IL1, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1B, IL1BETA, IL1β, interleukin 1 beta, Interleukin-1 beta