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Human Amyloid beta (aa1-40) Qu

antikine ELISA Kit,阿尔茨海默症科研检测试剂盒
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  • ¥6130
  • RD
  • 15.6 - 1,000 pg/mL
  • DAB140B
  • 阿尔茨海默症科研检测试剂盒
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 样本

      Cell Culture Supernates (200 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)

    • 标记物

      DAB140B

    • 适应物种

      Cell Culture Supernates (200 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)

    • 应用

      阿尔茨海默症科研检测试剂盒

    • 检测方法

      ELISA

    • 检测范围

      15.6 - 1,000 pg/mL

    • 供应商

      仅限科研使用,不得用于临床

    • 库存

      15.6 - 1,000 pg/mL

    • 规格

      96T

    Human Amyloid beta (aa1-40) Quantikine ELISA Kit,阿尔茨海默症科研检测试剂盒(仅限科研使用,不得用于临床)
    Assay Length
    4.5 hours
    Sample Type & Volume Required Per Well
    Cell Culture Supernates (200 uL), Tissue Lysates (10 uL), Cerebrospinal Fluid (10 uL)
    Sensitivity
    8.17 pg/mL
    Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid)
    Specificity
    Natural human Amyloid beta (aa1-40).
    Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
    Interference
    Interference observed with 1 or more available related molecules.

    Product Summary

    The Quantikine® Human Amyloid beta (aa1-40) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Amyloid beta (aa1-40) in cell culture supernates, tissue lysates, and cerebrospinal fluid. It contains synthetic human Amyloid beta (aa1-40) and antibodies raised against the synthetic peptide. Results obtained for naturally occurring human Amyloid beta (aa1-40) showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Amyloid beta (aa1-40).

    Precision

    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

    Cell Culture Supernates, Tissue Lysates, Cerebrospinal Fluid

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean (pg/mL) 58.9 264 556 56.3 247 518
    Standard Deviation 4.23 5.76 11.3 4.31 22.4 30.5
    CV% 7.2 2.2 2 7.7 9.1 5.9

    Recovery

    The recovery of human Amyloid beta spiked to levels throughout the range of the assay in various matrices was evaluated.

    Sample Type Average % Recovery Range %
    Cell Culture Media (n=4) 99 95-105
    Cell Lysis Buffer (n=2) 99 91-110
    Cerebrospinal Fluid (n=4) 104 96-113

    Linearity

    To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Amyloid beta were diluted with Diluent RD2-7 to produce samples with values within the dynamic range of the assay.
    Human Amyloid beta (aa 1-40) ELISA Linearity
    Human Amyloid  beta  (aa 1-40) ELISA Linearity

    Data Examples

    Human Amyloid beta (aa 1-40) ELISA Standard Curve

    Human Amyloid beta (aa 1-40) ELISA Standard Curve


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