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- ER1113
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- 2025年11月17日
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- 详细信息
- 文献和实验
- 技术资料
- 标记物：
78.125-5000pg/ml
- 供应商：
上海研卉生物
- 库存：
大量
- 规格：
96T
- Insulin ELISA Kit, INS ELISA Kit
- 适应种属
- Rat
- 检测范围
- 78.125-5000pg/ml
- 灵敏度
- 46.875pg/ml
- 产品名称
- Rat INS(Insulin) ELISA Kit
- 货号
- ER1113
- 别称
- Insulin ELISA Kit, INS ELISA Kit
- 检测方法
- Sandwich ELISA, Double Antibody
- 应用
- INS ELISA Kit allows for the in vitro quantitative determination of INS concentrations in serum, plasma, tissue homogenates and other biological fluids.
- 规格
- 48T/96T
- 检测范围
- 78.125-5000pg/ml
- 灵敏度
- < 46.875pg/ml
- 种属
- Rat
- UniProt ID
- P01322
- 标准曲线
-
- 存储
- 2-8 ℃ for 6 months
- 回收率
-
Matrices listed below were spiked with certain level of INS and the recovery rates were calculated by comparing the measured value to the expected amount of INS in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 86-100 91 EDTA plasma(n=5) 87-104 95 heparin plasma(n=5) 87-105 93 - 线性度
-
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of INS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 89-102% 86-103% 87-105% EDTA plasma(n=5) 84-97% 86-100% 92-100% heparin plasma(n=5) 80-99% 82-94% 80-96% - 变异系数（%）
-
批内差：CV<8%
批间差：CV<10% - 注意事项
- 仅供科研使用
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at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Genetic and Pharmacologic Models for Type 1 Diabetes
by a partial or total insufficiency of insulin. The premiere animal model of autoimmune T cell?mediated T1D is the NOD mouse. A dominant negative mutation in the mouse insulin 2 gene ( Ins2 Akita ) produces a severe insulin deficiency syndrome
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