胰蛋白酶抑制剂

产品编号 T6522 | CAS号 9035-81-8 | Sigma
胰蛋白酶抑制剂 来源于大豆

Trypsin inhibitor from Glycine max (soybean)
powder, BioReagent, suitable for cell culture
别名: SBTI

Components

The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.

Application

This product has been tested for cell culture applications. Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a ton-toxigenic mangrove isolate of Vibri sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.

Caution

A solution at 10 mg/mL should be stored for greater than 3 years at 2-8°C retained activity. Solutions are stable in frozen aliquots at -20°C.

Preparation Note

胰蛋白酶抑制剂可以 10mg/mL 或更高的浓度溶于水和磷酸盐缓冲液。浓度较高时溶液可能变浑浊，呈黄色至琥珀色。

Unit Definition

One trypsin unit = A₂₅₃ of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C. One mg will inhibit 1.0-3.0 mg of trypsin with activity of approximately 10,000 BAEE units per mg protein.

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Biochem/physiol Actions

大豆胰蛋白酶抑制剂可抑制胰蛋白酶活性，而很少抑制胰凝乳蛋白酶和纤溶酶活性。它可以 1:1 的化学计量与胰蛋白酶形成复合物。形成这种复合物后，胰蛋白酶可裂解抑制剂中的精氨酸-异亮氨酸单键。这种复合物的解离可生成改性或天然的抑制剂。在胰蛋白酶结合的最佳 pH 值 (pH 8.0) 下，缔合常数 ≥ 10×10⁸。

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