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Brilliant Violet 421™ anti-hum

an TCR α/β Antibody
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  • ¥5390
  • biolegend
  • C109598
  • 306722
  • 2025年07月14日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      上海研卉生物科技有限公司

    • 库存

      大量

    • 抗体英文名

      Brilliant Violet 421™ anti-human TCR α/β Antibody

    • 规格

      100T

    Brilliant Violet 421™ anti-human TCR α/β Antibody
    Clone
    IP26 (See other available formats)
    Regulatory Status
    RUO
    Other Names
    α/β TCR, TCR α/β
    Isotype
    Mouse IgG1, κ
    Ave. Rating
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    Product Citations
    6 publications
    Description

    The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.

    Product Details

    Product Details

    Isotype Control
    Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl
    Reactivity
    Human
    Antibody Type
    Monoclonal
    Host Species
    Mouse
    Formulation
    Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
    Preparation
    The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
    Concentration
    Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
    Storage & Handling
    The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
    Application

    FC - Quality tested

    Recommended Usage

    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

    Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


    Learn more about Brilliant Violet™.

    This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
    Excitation Laser
    Violet Laser (405 nm)
    Application Notes

    Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. Other anti-CD3 clones may compete out the binding of IP26.

    Application References

    (PubMed link indicates BioLegend citation)
    1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC)
    2. Joseph A, et al. 2008. J. Virol. 82:3078. (FC) PubMed
    3. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
    Product Citations
    1. Goel G, et al. 2019. Sci Adv. 5:eaaw7756. PubMed
    2. Mayassi T, et al. 2019. Cell. 176:967. PubMed
    3. Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed
    4. Barbarin A, et al. 2017. Front Immunol. 8:316. PubMed
    5. Schreurs RRCE, et al. 2021. Mucosal Immunology. 14(3):605-614. PubMed
    6. Hamilton JR, et al. 2021. Cell Reports. 35(9):109207. PubMed
    RRID
    AB_2562077 (BioLegend Cat. No. 306721)
    AB_2562805 (BioLegend Cat. No. 306722)
    Brilliant Violet 510™ anti-human TCR γ/δ
    品牌Biolegend
    货号331220
    规格100 tests
    CloneB1

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