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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海研卉生物科技有限公司
- 库存:
大量
- 抗体英文名:
Brilliant Violet 421™ anti-human TCR α/β Antibody
- 规格:
100T
- Clone
- IP26 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α/β TCR, TCR α/β
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- 6 publications
Description
The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.
Product DetailsBrilliant Violet 510™ anti-human TCR γ/δProduct Details
- Isotype Control
- Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone. Other anti-CD3 clones may compete out the binding of IP26.
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2562077 (BioLegend Cat. No. 306721)
AB_2562805 (BioLegend Cat. No. 306722)
品牌Biolegend
货号331220
规格100 tests
CloneB1
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文献和实验1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法: 货号 名称
. Fig. 5.1. Inhibition of GSK-3 activity. A Depletion of both GSK-3α and GSK-3β is required for a decrease of β-catenin phosphorylationat Ser33/37. Panc04.03 cells were transfected with shControl, shGSK-3α, and shGSK-3β vectors, as describedin
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