- ¥1650
- GIBCO
- 12200036
- GIBCO货号：12200-036
- 2025年09月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
上海研卉生物科技有限公司
- 规格：
10X1L
描述
Iscove's Modified Dulbecco's Medium (IMDM) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a variety of Gibco® IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This IMDM is modified as follows:
| With | Without |
| • L-glutamine | • α-thioglycerol |
| • Phenol Red | • 2-mercaptoethanol |
The complete formulation is available.
IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® IMDM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® IMDM product made in our Scotland facility (42200-014). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
规格
| Glutamine: | L-Glutamine |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Powder |
| HEPES Buffer: | HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Glucose: | High Glucose |
| Product Line: | Gibco® |
| Product Size: | 10 x 1 L |
| Shipping Condition: | Room Temperature |
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文献和实验40 维生素 B 12 0.005 RPMI-1640(A) 无酚红细胞培养基（粉末型）成分
实验大纲 骨髓间充质干细胞是多能干细胞。因此，作为再生医学的细胞来源，它是活性研究和临床应用的目标。当骨髓液或骨髓细胞接种在培养皿上时，间充质干细胞会作为粘附细胞增殖。因此，间充质干细胞可以从漂浮的血细胞中分离出来。在此，我们报告了使用 Evident Prov CM20 培养监测系统对间充质干细胞原代培养的远程监测结果。 试验程序 使用补充了 10% 胎牛血清（FBS）和 1% 青霉素链霉素的 Iscove 改良 Dulbecco 培养基（IMDM），将市售人骨髓细胞以 950,000
.43Kpa灭菌20分钟，取出后趁热将试管倾斜一定角度放置，待琼脂 凝固即成琼脂斜面培养基(图3)。三角烧瓶内培养基冷至50-60℃时在超净台或无菌室内将其倾注于灭菌平皿内(15-20ml)，凝固后即成琼脂平板。 图： 琼脂斜面培养基 另外，实际工作中现多使用合成营养琼脂干燥粉末制剂。其方法为：称取41克营养琼脂干燥粉末加入1000ml冷蒸馏水中混合放置10分钟，隔石棉铁丝网微火煮沸使其完全溶解，分装于中试管中(约5~7ml)或三角烧瓶，103.43kPa
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