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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海研卉生物科技有限公司
- 库存:
500-P49BT
- 靶点:
TPO
- 目录编号:
500-P49BT
- 抗原来源:
18.6 kDa Recombinant Human TPO (PeproTech catalog# 300-18)
- 抗体英文名:
生物素标记Anti-Human TPO
- 抗体名:
生物素标记Anti-Human TPO
- 标记物:
生物素标记
- 宿主:
兔
- 适应物种:
人
- 亚型:
18.6 kDa Recombinant Human TPO (PeproTech catalog# 300-18)
- 应用范围:
WB ELISA
- 浓度:
生物素标记Anti-Human TPO
- 规格:
25ug
Source: Polyclonal Rabbit
Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTPO. Anti-Human TPO specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen: E.coli-derived, 18.6 kDa Recombinant Human TPO (PeproTech catalog# 300-18)
Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human TPO (500-P49) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hTPO.
Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
| 500-P49-50 | Rabbit Anti-Human TPO | 50ug |
| 500-P49-100 | Rabbit Anti-Human TPO | 100ug |
| 500-P49-1000 | Rabbit Anti-Human TPO | 1000ug |
| 500-P49Bt-25 | Biotinylated Rabbit Anti-Human TPO | 25ug |
| 500-P49Bt-50 | Biotinylated Rabbit Anti-Human TPO | 50ug |
| 500-P49Bt-1000 | Biotinylated Rabbit Anti-Human TPO | 1000ug |
| 500-P49G-50 | Goat Anti-Human TPO | 50ug |
| 500-P49G-100 | Goat Anti-Human TPO | 100ug |
| 500-P49G-1000 | Goat Anti-Human TPO | 1000ug |
| 500-P49GBt-25 | Biotinylated Goat Anti-Human TPO | 25ug |
| 500-P49GBt-50 | Biotinylated Goat Anti-Human TPO | 50ug |
| 500-P49GBt-1000 | Biotinylated Goat Anti-Human TPO | 1000ug |
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文献和实验。 9. 最后,样品加入叠氮钠(终浓度0.5 g/L)及1.0 g/L BSA。将结合产物置4℃,避光保存,亦可加入50%重蒸甘油,置-20℃保存。 要点: 1. 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对0.1 mol/L 碳酸氢钠缓冲液或0.5 mol/L 硼酸缓冲液 2. 所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件。
步骤 1. 在一支灭菌的Eppendof离心管中加入20μl探针DNA(0.5-1μg/μl,溶解于水中 2. 避光条件下加等体积的光敏生物素醋酸盐并混匀。 3. 将装有反应液的离心管置于冰模中,暗室中在光标记灯下照射30分钟(灯距样品15cm)。 4. 加入无菌水至总体积为100μl 5. 加入100μl仲丁醇,混匀。10000rpm离心10min,去上层液,再加入仲丁醇,离心,使水相浓缩至30
1ml/管,蛋白质在1-3ml之间洗下; 9.最后,样品加入叠氮钠(终浓度0.5g/L)及1.0g/L BSA。将结合产物置4℃,避光保存,亦可加入50%重蒸甘油,置―20℃保存。 要点: 1. 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对0.1 mol/L碳酸氢钠缓冲液或0.5 mol/L硼酸缓冲液充分透析; 2. 所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个
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