LS-F40046 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Beta Endorphin in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Beta Endorphin as low as 9.375 picograms per milliliter.
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Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Coated 96-well Strip Plate
Adhesive Plate Sealers
Sample Diluent
Wash Buffer (25x)
TMB Substrate
Stop Solution
Standard (Lyophilized)
Biotinylated Detection Antibody (100x)
HRP Conjugate Diluent
Biotinylated Detection Antibody Diluent
HRP Conjugate (100x)
Background
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated withBetaEP. During the reaction,BetaEP in the sample or standard competes with a fixed amount ofBetaEP on the solid phase supporter for sites on the Biotinylated Detection Antibody specific toBetaEP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration ofBetaEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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