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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海研卉生物科技有限公司
- 库存:
大量
- 规格:
100ml
描述
Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture-treated plastic and glass surfaces. Poly-D-Lysine contains polymers with a molecular weight of 50,000–150,000 Daltons, which makes this product ideal for neuronal culture applications. This ready-to-use product is provided as a liquid at a concentration of 0.1 mg/mL. When used on Nunc cell culture plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.Key features include:
• Synthetic and animal origin-free
• Sterile-filtered for cell culture applications
• Validated with B-27 Supplement and B-27 Plus Neuronal Culture System when used with Nunc plastic and glass bottom dishes
规格
| Classification: | Animal Origin-Free |
|---|---|
| Format: | Bottle(s) |
| Product Line: | Gibco™ |
| Product Size: | 100 mL |
| Purity or Quality Grade: | Cell Culture Grade |
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文献和实验POLY-L-LYSINE COATED SLIDES SOLUTIONS --5% Chromic acid --0.01% Poly-L-Lysine (PLL) - made from a dry protein, with distilled water. (100mg/1 litre) This procedure can be carried out on either a ski or carousel staining
多聚赖氨酸(Poly-L-Lysine)的配制、保存、使用总结
用多聚赖氨酸涂片培养细胞,不知道多聚赖氨酸涂过的玻片如何灭菌?能否干烤灭菌?答:Sigma的产品说明书上写的很明白的。另外,有本书上是这么写的,供参考:Poly-lysine-coated tissue culture surfacesTo coat coverslips: Prepare a stock solution by dissolving 25 mg/ml polylysine in 4.73 ml water (both poly-L-lysine and poly-D-lysine
Silane and Poly-L-Lysine Coating of Microscope Slides
free environment for 5 minutes. Poly-L-Lysine Coating a) Smear 20ul of poly-l-lysine over each slide. b) Air dry in a dust free environment. Silated Slides a) To 300mls acetone add 6mls Aminopropyltriethoxysilane solution (Sigma
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