LS-F9113 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human BLK in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of BLK as low as 0.071 nanograms per millilter.
About BLK
Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. P51451NM_001715NP_001706.2
BLK ElisaKit, Blk kinase ElisaKit, B lymphocyte kinase ElisaKit, B lymphoid tyrosine kinase ElisaKit, MODY11 ElisaKit, Protein kinase blk ElisaKit, Protein tyrosine kinase blk ElisaKit, B lymphoid kinase ElisaKit, p55-Blk ElisaKit, Tyrosine-protein kinase Blk ElisaKit
B-Lymphoid Tyrosine Kinase (BLK) No significant cross-reactivity or interference between B-Lymphoid Tyrosine Kinase (BLK) and analogs was observed.
Manual
Intended Sample Types
Cell Lysates, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Detection Range
0.156 - 10 ng/ml
Sensitivity
0.071 ng/ml
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Coated 96-well Strip Plate
Adhesive Plate Sealers
Standard (Lyophilized)
Sample Diluent
Detection Reagent A (Green)
Detection Reagent B (Red)
Assay Diluent A
Assay Diluent B
TMB Substrate
Stop Solution
Wash Buffer (30x concentrate)
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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