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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Bcl-2抗体
- 库存:
大量
- 抗体英文名:
BCL2 Antibody
- 是否单克隆:
0
- 保质期:
一年
- 适应物种:
human, Astragalus membranaceus, Bovine, chicken, cow, guinea pig, N. guentheri, pig, rabbit, sheep
- 应用范围:
WB, IP, IHC, IF, FC, chIP, ELISA
- 宿主:
Rabbit
- 供应商:
上海研卉生物科技有限公司
- 保存条件:
低温
- 规格:
100ul
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Showing 1- [6] of [851] Publications
| Author: | Xiaodong Xue | |
| Pubmed ID: | 28769046 | |
| Journal: |
Sci Rep
|
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| Title: |
Kaiso protects human umbilical vein endothelial cells against apoptosis by differentially regulating the expression of B-cell CLL/lymphoma 2 family members.
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| Application: | WB,chIP | |
| KD validated | ||
| Species: | human | |
| Author: | Shizan Xu | |
| Pubmed ID: | 28774703 | |
| Journal: |
Life Sci
|
|
| Title: |
Pretreatment with propylene glycol alginate sodium sulfate ameliorated concanavalin A-induced liver injury by regulating the PI3K/Akt pathway in mice.
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|
| Application: | WB,IHC | |
| Species: | mouse | |
| Author: | Yanli Liu | |
| Pubmed ID: | 28779179 | |
| Journal: |
Sci Rep
|
|
| Title: |
mTORC1 regulates apoptosis and cell proliferation in pterygium via targeting autophagy and FGFR3.
|
|
| Application: | WB,IHC | |
| Species: | human | |
| Author: | Fengzhi Chen | |
| Pubmed ID: | 28781215 | |
| Journal: |
J Sex Med
|
|
| Title: |
Adipose-Derived Stem Cell-Derived Exosomes Ameliorate Erectile Dysfunction in a Rat Model of Type 2 Diabetes.
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|
| Application: | WB | |
| Species: | rat | |
| Author: | Hao Tang | |
| Pubmed ID: | 28790461 | |
| Journal: |
Sci Rep
|
|
| Title: |
Anti-colon cancer effect of caffeic acid p-nitro-phenethyl ester in vitro and in vivo and detection of its metabolites.
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|
| Application: | WB | |
| Species: | human | |
| Author: | Jichao Wu | |
| Pubmed ID: | 28796243 | |
| Journal: |
Cell Death Dis
|
|
| Title: |
Exogenous H2S facilitating ubiquitin aggregates clearance via autophagy attenuates type 2 diabetes-induced cardiomyopathy.
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|
| Application: | WB | |
| Species: | mouse | |
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文献和实验【求助】如果要证明Bcl-2和RARP活性,应该做什么实验?
eeflying 目前有两种化合物,分别为Bcl-2和PARP的抑制剂, 虽然这两个指标通常作为细胞凋亡的指标常用于分子生物实验, 但是,如何证明这两个分子被抑制的实验却第一次碰到, 请各位战友支招,我要直接检测哪个蛋白的WB 或者做什么实验合适? magichunter 用western检测bcl-2的含量变化,检测PARP活性裂解片段的含量变化以及原型态PARP的含量
Bcl-2蛋白是bcl-2 原癌基因的编码产物,是细胞存活促进因子,属膜整合蛋白,分子量为26kDa, 定位于线粒体、内质网和连续的核周膜。Bcl-2蛋白家族是一个特别的家族,成员中有些促进凋亡,如Bad、Bid 、Bax,有些成员阻止细胞凋亡,如Bcl-2、 Bcl-x、Bcl-w。Bcl-2能够阻止细胞色素c从线粒体释放到细胞质,从而抑制了细胞凋亡。
抗体的结构决定了其对应的功能,同一抗体的V区和C区的氨基酸组成和顺序不同,决定了其功能上的差异。V区和C区的组成和结构,决定了抗体的生物学功能。 图1:抗体的主要功能(图片来源:Abbas et al: Cellular and Molecular Immunology, 7e.) 1、特异性识别功能 抗体可以通过其结构上的可变区域与抗原结合,同时,具有极高的特异性。这种特异性识别功能可以使抗体识别和结合到感染的病原体,肿瘤细胞等不同种类的分子,帮助免疫系统识别并定位到这些分子,进而启动
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