IQ-1 enhances β-catenin/CBP interactions and blocks β-catenin/p300 driven transcription, which enables mouse embryonic stem cells (ESCs) to be maintained in an undifferentiated pluripotent state,IQ-1 is a cell-permeable tetrahydroisoquinolinylidene compound that modulates Wnt/β-catenin signaling. Targets the PR72/130 subunit of PP2A. Thereby blocking PP2A/Nkd complex formation, resulting in diminished β-catenin/p300 interaction and a concomitant increase in β-catenin/CBP usage. Co-administration of IQ-1 (4 μg/ml) and Wnt3a (100 ng/ml), but neither reagent alone, has been shown to be sufficient in maintaining long-term murine Embryonic Stem Cells pluripotency in the absence of serum. allowing expansion and self-renewal of mouse ESC populations.