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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Rat
96T
货号 | EK0390 |
---|---|
产品名称 | Rat IL-1 alpha ELISA Kit |
产品描述 | For quantitative detection of rat IL-1 alpha in cell culture supernates and serum. |
免疫原 | E.coli,S115-S270 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.5pg/ml |
检测范围 | 4.69pg/ml-300pg/ml |
基因名称 | IL1A |
基因全名 | interleukin 1, alpha |
Uniprot ID | P16598 |
指标别名 | Hematopoietin 1|IFL|IFN|IFN alpha|IFN-alpha-1/13|IFNA@|IFNA1|IFNA1_HUMAN|IFNA13|IFNAP22|IL 1 alpha|Il 1a|IL1|IL1 ALPHA|IL1A|IL1F1|Ilia|Interferon Alpha|Interferon alpha 1|Interferon alpha 1/13|Interferon alpha 1/13 precursor|Interferon alpha 13|Interferon alpha D|Interferon alpha leukocyte|Interferon alpha-1/13|Interferon alpha-D|Interferon leukocyte|Interleukin 1 alpha|Interleukin 1 alpha precursor|Interleukin1 alpha|LeIF D|MGC138207|MGC138505|MGC138507|OTTHUMP00000203692|OTTMUSP00000016430|Preinterleukin 1|Pro interleukin 1|Pro interleukin 1 alpha|Prointerleukin 1|RP23-160G19.8 |
背景资料 | Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18kDa. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0390-CAP | 96-well plate precoated with anti- rat IL-1 alpha antibody | 1 |
EK0390-ST | Lyophilized recombinant rat IL-1 alpha standard | 10ng/tubex2 |
EK0390-DA | Biotinylated anti- rat IL-1 alpha antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 15 | 40 | 131 | 14 | 43 | 120 |
Standard deviation | 1.06 | 2.12 | 9.69 | 1.09 | 2.58 | 10.8 |
CV(%) | 7.1% | 5.3% | 7.4% | 7.8% | 6% | 9% |
Concentration(pg/ml) | 0 | 4.69 | 9.4 | 18.8 | 37.5 | 75 | 150 | 300 |
O.D. | 0.040 | 0.110 | 0.157 | 0.306 | 0.588 | 1.013 | 1.552 | 2.148 |
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