- $815
- LSBio
- 15.6 - 1000 ng/ml
- LS-F10838
- 美国
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 样本：
血清/血浆/组织匀浆液
- 库存：
1000
- 适应物种：
Human
- 应用：
科研使用
- 检测方法：
ELISA
- 检测范围：
15.6 - 1000 ng/ml
- 供应商：
康朗生物
- 规格：
96T
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Product Description
LS-F10838 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Antithrombin-III in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Antithrombin-III as low as 15.6 nanograms per millilter.About Antithrombin-III
SERPINC1 / Antithrombin III is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. P01008 NM_000488NP_000479.1
SERPINC1 ElisaKit, Antithrombin III ElisaKit, Antithrombin-III ElisaKit, ATIII ElisaKit, AT3 ElisaKit, Serpin C1 ElisaKit, AT3D ElisaKit, THPH7 ElisaKit
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Specifications
TypeSandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
Antithrombin-III
Reactivity
Human
Specificity
Recognizes human ATIII. No significant cross-reactivity or interference was observed.
Manual
Intended Sample Types
Plasma, Serum
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
15.6 - 1000 ng/ml
Sensitivity
15.6
Precision
Intra-Assay: CV<10% / Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated 96-well Strip Plate
- Standard (Lyophilized)
- Sample Diluent
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Wash Buffer (25x concentrate)
- TMB Substrate
- Stop Solution
- Adhesive Plate Sealers
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Sandwich ELISA Platform Overview
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
）的Human IL6 ELISA READY-SET-GO 盒，商家推荐操作流程为：1用包被抗体包被酶联板（4度过夜）→2洗三遍（第I洗）→3加入阻断缓冲液（通常为含10%小牛血清或5%脱脂奶粉的洗液），室温下放置1小时→4洗三遍（第II洗）→5加入样本，室温下放置1小时→6洗五遍（第III洗）→7加入检测抗体（生物素标记），室温下放置1小时→8洗五遍（第IV洗）→9加入亲合素标记的HRP反应液，室温下放置半小时→10洗七遍（第V洗）→11加入酶底物显色→12终止反应→13用酶联仪读板。在此流程中前后共有
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