- 询价
- BIO-RAD
- 3564748
- 2025年11月17日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
100g,1.25L
Use RAPID'E.coli O157:H7 Agar for the detection, isolation, and presumptive identification of E. coli O157:H7 in food products. Results should be confirmed by standard reference methods appropriate for the food type being tested. Confirmation using latex is also validated for colonies isolated from RAPID'E.coli O157:H7 agar.
This product includes 100 g of dehydrated medium. Supplements of novobiocin (10 mg/L) and potassium tellurite (0.8 mg/L) must be added to this medium.
Features and Benefits
- Detection on a single plate
- Typical (sorbitol-/β-glucuronidase-) and atypical (β-glucuronidase+) E. coli O157:H7 colonies are dark blue or black
- Other atypical (sorbitol+) E. coli O157:H7 colonies are blue to turquoise blue
- 100% specificity demonstrated by testing 50 strains of E. coli O157:H7 and 35 other strains
- Selectivity is increased by the addition of novobiocin and other selective agents such as potassium tellurite
- Results with RAPID'E.coli O157:H2 Medium in ≤48 hr
Validations
Certified NF VALIDATION according to the ISO 16140 standard and validated by the AOAC.
Related Products
- STEC Enrichment Broth, dehydrated, 500 g (3564001) and 5 kg (3564002)
- Modified Tryptic Soy Broth (mTSB) + Novobiocin, ready-to-use (3555426)
- Modified Tryptic Soy Broth (mTSB) Base, dehydrated (3564426)
- Novobiocin, powder (3564610)
- iQ-Check® E. coli O157:H7 Real-Time PCR Kit (3578114)
- iQ-Check STEC VirX Real-Time PCR Kit (3578139) for detection of stx1-stx2 and eae virulence genes
- iQ-Check STEC SerO Real-Time PCR Kit (3578140) for detection of six O serotypes (O26, O111, O45, O145, O121, and O103) plus E. coli O157:H7
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
In recent years several techniques have been described for the introduction of DNA molecules into Escherichia coli . These are based on the findings of Mandel and Higa (1 ), who demonstrated that incubation of cells with naked bacteriophage DNA
1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10',5K,4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃ 5.Wash 1X 40 ml 10% glycerol--> transfer to S34 tubes & spin
技术资料暂无技术资料 索取技术资料
