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- 文献和实验
- 技术资料
- 库存：
1000
- 现货状态：
现货
- 供应商：
杭州欣友生物技术有限公司
- 规格：
8个/包，48个/箱
一、8孔腔室盖玻片：
玻底培养皿和玻底培养板主要用于要求放大倍数高、培养器皿底面透光性能好的显微实验，如激光共聚焦显微实验、荧光显微实验和相差显微实验等。玻底培养皿和玻底培养板底面薄，透光性能好， 满足了上述实验的要求。同时玻底培养皿和玻底培养板底面的小孔也减少了实验过程中抗体等试剂的使用量。
Cellvis公司为生物研究人员提供优质廉价的玻底皿和玻底板。Cellvis公司的玻底皿和玻底板底面玻璃使用优质玻片，产品采用无细胞毒性的医用胶水粘合，适用于激光共聚焦等需要高分辨率的细胞显微实验，并且能够耐受长期的细胞培养。
8孔腔室盖玻片使用专门的模具生产，产品均一，使用便利。塑料板使用高透明度的USP class VI 聚苯乙烯为原料制成。产品适合贴壁细胞的培养。产品在无尘车间中生产，保证洁净度。
二、产品优势：
- 使用进口优质玻片，特别适合激光共聚焦实验
- 使用进口USP class VI无细胞毒性的胶水
- 在无尘车间内生产处理和包装
- 通过细胞生物学相关实验的质量检测
- 无热原(<0.5EU/ml)
- Ɣ射线辐照灭菌
三、包装规格
| 产品号 | 品牌 | 包装规格 |
| C8-1.5H-N | Cellvis | 8格，1.5号高平玻片，8个每包，48个每箱。 |
*产品玻片厚度为0.17±0.005mm。
四、玻底培养皿和玻底培养板使用方法(以35mm皿，10mm孔为例)
- 预平衡：在玻底培养皿加入3ml培养液，在培养箱中放置15分钟。
- 加细胞：吸去培养液，在底孔中加入500ul含细胞的培养液。在培养箱中放置2小时，让细胞沉降贴壁。
- 加培养液：小心加入2-3ml不含细胞的培养液。该步骤用于为细胞提供足够的培养液，同时减少由于水份挥发带来的渗透压的变化。
*根据实验的要求，可以合并步骤2和3，在预平衡后直接加入2-3毫升含细胞的培养液。
五、产品尺寸图
六、该产品被引用文献
- EWI-2 Inhibits Cell-Cell Fusion at the Virological Presynapse
Emily E Whitaker, et al., bioRxiv - Microbiology 2019, published in Viruses doi: 10.3390/v11121082
Quote: ... seeded onto 8-well glass-bottom plates (CellVis, Mountain View, CA, Cat. #C8-1.5H-N) coated with 1:10 poly-L-Lysine in double-distilled water (ddH2O) ... - The dynamic Nexus: Gap junctions control protein localization and mobility in distinct and surprising ways
Sean McCutcheon, et al., bioRxiv - Cell Biology 2020
Quote: ... For standard FRAP experiments N2A and HeLa cells were plated into 8-well imaging chambers (iBidi, cat no. 80826 or In Vitro Scientific, C8-1.5H-N) and each well was transfected with 0.5 μg of each plasmid to drive expression of Connexin-fluorescent protein fusions 24-72 h prior to imaging ...
七、企业资质
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文献和实验- Synergistic block of SARS-CoV-2 infection by combined drug inhibition of the host entry factors PIKfyve kinase and TMPRSS2 protease
Alex J.B. Kreutzberger, et al., bioRxiv - Microbiology 2021
Quote: ... 25 mm (Cellvis cat. C8-1.5H-N), Polydimethylsiloxane (PDMS ... - Transcription regulates bleb formation and stability independent of nuclear rigidity
Isabel K. Berg, et al., bioRxiv - Cell Biology 2022
Quote: ... Cells were plated in 8 well coverslips (Cellvis, C8-1.5H-N) grown and left untreated or treated with VPA and/or alpha amanitin ... - Self-assembly and structure of a clathrin-independent AP-1:Arf1 tubular membrane coat
Richard M. Hooy, et al., bioRxiv - Cell Biology 2022
Quote: ... cells were plated onto 8 well chambered cover glass (Cellvis C8-1.5H-N) and incubated with 100nM JF635 dye ... - Multiple-labeled antibodies behave like single emitters in photoswitching buffer
Dominic A. Helmerich, Gerti Beliu, Markus Sauer, bioRxiv - Biophysics 2020
Quote: ... 8 chambered cover glass systems with high performance cover glass (Cellvis, C8-1.5H-N) were used ... - Molecular Crowding Facilitates Bundling of IMPDH Polymers and Cytoophidium Formation
Chia-Chun Chang, et al., bioRxiv - Cell Biology 2022
Quote: OFP-IMPDH2 expressing HeLa cells on glass bottom chamber slides (C8-1.5H-N, Cellvis) with medium containing 100 μM MPA (M3536 ... - EWI-2 Inhibits Cell-Cell Fusion at the HIV-1 Virological Presynapse
Emily E. Whitaker, et al., bioRxiv - Microbiology 2019
Quote: ... seeded onto 8-well glass-bottom plates (CellVis, Mountain View, CA, Cat. #C8-1.5H-N) coated with 1:10 poly-L-Lysine in double-distilled water (ddH2O) ... - Six-Dimensional Single-Molecule Imaging with Isotropic Resolution using a Multi-View Reflector Microscope
Oumeng Zhang, et al., bioRxiv - Biophysics 2022
Quote: ... we plate HEK-293T cells at 50% confluency on 8-chamber coverglass (Cellvis C8-1.5H-N) treated with fibronectin (SigmaAldrich FC010) ... - The X-linked intellectual disability gene product and E3 ubiquitin ligase KLHL15 degrades doublecortin proteins to constrain neuronal dendritogenesis
Jianing Song, et al., bioRxiv - Biochemistry 2020
Quote: Primary rat hippocampal cultures from E18 embryos were plates at 50,000 cells/ml in cover glass bottomed chambers and plates (C8-1.5H-N and P24-1.5H-N, Cellvis). At DIV 2 ... - Super-resolution imaging reveals dynamic reticular cytoophidia
Yifan Fang, Yi-Lan Li, Xiao-Ming Li, Ji-Long Liu, bioRxiv - Biophysics 2022
Quote: 293T cells transfected with hCTPS1-mCherry and hCTPS1-miRFP670nano constructs were cultured on glass bottom culture dishes (C8-1.5H-N, Cellvis) with medium and maintained at 37 °C when live imaging was performed. - Engineered molecular sensors of cell surface crowding
Sho C. Takatori, et al., bioRxiv - Biophysics 2022
Quote: PBS solution containing unconjugated WGA was incubated into an 8-well chambered cover glass (Cellvis; catalog number: C8-1.5H-N) to coat the glass with WGA ...
Cited publications before 2019 (11)
-
The effects of IKK-beta inhibition on early NF-kappa-B activation and transcription of downstream genes
MJ Bloom, et al., Cellular Signalling Volume 55, March 2019, Pages 17-25
Quote: "One day before the stimulation experiments, cells were seeded in 8-well chambered cover glass (Cellvis, Mountain View, CA) in 200 μl of culture medium at an estimated 35,000 cells/well." -
The AP-1 complex regulates AXL expression and determines sensitivity to PI3Kα inhibition in esophagus and head and neck squamous cell carcinoma
Mai Bdarny, et al., bioRxiv, October 08, 2018
Quote: "For IF, cells were seeded on 8-well glass slides (Cellvis, Cat no. C8-1.5H-N) for 48 hours" -
Visualization of Calcium Ion Loss from Rotavirus during Cell Entry
Eric N. Salgado, et al., Journal of Virology, 2018
Quote: "For live-cell imaging, BSC-1 cells were grown on 8-chambered number 1.5 cover glass slides (Cellvis) in a volume of 400 μl supplemented DMEM and allowed to grow overnight to 50% confluence." -
EWI-2 Inhibits Cell-Cell Fusion at the Virological Synapse
EE Whitaker, et al., BioRxiv, April 06, 2018
Quote: "Three days post infection, 3 × 10 5 infected cells were plated onto each well of 8-well glass-bottom plates (CellVis, Mountain View, CA, Cat. #C8-1.5HN) coated with 1:10 poly-L-Lysine in ddH2O" -
Quantitative Super-Resolution Imaging of Small RNAs in Bacterial Cells
S Park, et al., Bacterial Regulatory RNA pp 199-212
Quote: "The imaging buffer: 10 mM NaCl, 50 mM Tris and 10% glucose in 1× PBS (for immunostained samples) or 4× SSC (for FISH samples) , with pH adjusted to 8. 5. 8-Well chambered cover glass (Nunc Lab-Tek 155409 or Cellvis C8-1.5HN)" -
Analysis of microtubule dynamics heterogeneity in cell culture
Anara Serikbaeva, et al., Methods in Molecular Biology, Cellular Heterogeneity pp 181-204
Quote: "For life imaging of transfected cells, we used eight-chambered cover glass system #1.5 high-performance cover glass (0.17 ± 0.005 mm) (In Vitro Scientific—www.invitrosci.com)" -
Size-dependent segregation controls macrophage phagocytosis of antibody-opsonized targets
Matthew H Bakalar, et al., BioRxiv, January 19, 2018
Quote: "For imaging interfaces between cells and supported lipid bilayer coated beads, cells were seeded into 8-well imaging chambers with a cover-glass bottom (Cellvis) and beads were added to the wells once the cells had fully adhered to the cover-glass" -
A Förster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells
BAI Min, et al., Chinese Journal of Analytical Chemistry Volume 46, Issue 1, January 2018, Pages 39-47
Quote: "Cell imaging analysis of H2S in living cells using QDS-N3 ratiometric probe HeLa and MCF-7 cells were seeded in a 8-well Chambered Cover Glass (Cellvis, USA) at 40000 cells per well at 37 ºC for overnight." -
Fabricating MnO2 Nanozymes as Intracellular Catalytic DNA Circuit Generators for Versatile Imaging of Base‐Excision Repair in Living Cells
F Chen, et al., Advanced Function Materials, 2017
Quote: "HeLa cells and MCF-7 cells were seeded in 8-well Chambered Cover Glass (Cellvis, USA) at 60 000 cells per well at 37 °C for overnight" -
IKKα regulates human keratinocyte migration through surveillance of the redox environment
Thomas S. Lissa, et al., Journal of Cell Science
Quote: "For RNA and scratch analyses, cells were cultured in 12-well plates (Corning, 3513) and 8-chamber glass-bottom dishes (In Vitro Scientific, C8-1.5H-N), respectively."
血细胞计数器（也被拼写血球计数仪）是一种蚀刻玻璃室提出双方将举行石英盖玻片完全室地板0.1毫米以上。为9毫米2的总表面积的计数室中进行蚀刻。 图1、 血细胞计数器的尺寸。 浓度的计算是根据上下方的盖玻片的体积。一个大广场（参见图2中的W）拥有量为0.0001毫升（长x宽x高，也就是说，0.1厘米×0.1厘米×0.01厘米）。 在这两种方法中，通过毛细管作用填充血细胞计数器 - 放置填充的吸移管具有良好的悬吊在血细胞计数器的边缘的凹口的细胞混合，然后慢慢地排出
，因此务必使用中心区域的孔，并避免使用边缘附近的孔影响稳定性。比如说，使用的是 96 孔板（12×8 孔），就只使用中心的 60 孔（10×6 孔）进行实验。 图 1.载物台上式培养箱 2.通过创造稳定的环境温度改善聚焦效果 需要考虑的因素是室内温度，细微的温度变化也会影响到成像性能。 显微镜的环境温度稳定性对于活细胞的高倍率观察尤其重要。由于高数值孔径观察的景深较浅，因此即使温度变化造成很小的Z漂移，系统也可能会因此离焦。 为了优化环境条件，开始实验之前请确保打开空调并保持室温稳定。另外为了提高
引言本实验所用基因传递系统（基因枪）原理： 低压基因递送系统（GDS-80 基因枪 U.S. Patent Number: 6,436,709 B1），根据火箭喷嘴原理和空气动力学原理设计，是用于传递生物微粒进入靶细胞的一种新型系统。如图1中所示，当左侧出现输入气体压力时（如：氦气），两个腔室之间将形成巨大的压力差，一旦该气体穿过装载样品的咽喉处，GDS-80 中的气体输出将为生物粒子提供足够的动能量，使它们加速到接近超音速的速度（约 300m/s）。在加速过程中，样品溶液会雾化并均匀
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