存储温度:The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
存储溶液:The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
参考文献:1. Katso, R. M. et.al: Functional analysis of H-Ryk, an atypical member of the receptor tyrosine kinase family. Molec. Cell. Biol. 19: 6427-6440, 1999. 2. Lu, W. et.al: Mammalian Ryk is a Wnt coreceptor required for stimulation of neurite outgrowth. Cell 119: 97-108, 2004.
简单描述:RYK is a pool of three individual synthetic siRNA duplexes designed to knock-down human RYK mRNA expression. Each siRNA is 19-25 bases in length.
Description: RYK is a pool of three individual synthetic siRNA duplexes designed to knock-down human RYK mRNA expression. Each siRNA is 19-25 bases in length.
Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.