存储温度:The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
存储溶液:The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
参考文献:1. Wang, M.H. et al: Identification of the ron gene product as the receptor for the human macrophage stimulating protein. Science, 1994; 266 (5182): 117-9 2. Li, B,Q, et al: "Macrophage-stimulating protein activates Ras by both activation and translocation of SOS nucleotide exchange factor. Biochem. Biophys. Res. Commun.1995; 216 (1): 110-8.
简单描述:RON is a pool of three individual synthetic siRNA duplexes designed to knock-down human RON mRNA expression. Each siRNA is 19-25 bases in length.
Description: RON is a pool of three individual synthetic siRNA duplexes designed to knock-down human RON mRNA expression. Each siRNA is 19-25 bases in length.
Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.