存储温度:The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
存储溶液:The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
参考文献:1. Hartmann, T. N. et.al: Human B cells express the orphan chemokine receptor CRAM-A/B in a maturation-stage-dependent and CCL5-modulated manner. Immunology 125: 252-262, 2008. 2. Arenzana-Seisdedos, F. et.al: HIV blocked by chemokine antagonist. (Letter) Nature 383: 400 only, 1996.
商品关键词:RANTES (CCL5) siRNA Set I,,R801-911-05,SignalChem
简单描述:RANTES (CCL5) is a pool of three individual synthetic siRNA duplexes designed to knock-down human RANTES (CCL5) mRNA expression. Each siRNA is 19-25 bases in length.
Description: RANTES (CCL5) is a pool of three individual synthetic siRNA duplexes designed to knock-down human RANTES (CCL5) mRNA expression. Each siRNA is 19-25 bases in length.
Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.