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PKC beta II siRNA Set I

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  • ¥29261
  • SignalChem
  • 加拿大
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      安诺伦(北京)生物科技有限公司

    • 规格

      50 nmol

    研究领域:Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases,

    存储温度:The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.

    存储溶液:The siRNAs are supplied as a lyophilized powder and shipped at room temperature.

    参考文献:1. Greenham, J. et al: Elucidation of the exon-intron structure and size of the human protein kinase C beta gene (PRKCB). Hum. Genet. 103: 483-487, 1998. 2. Su, T T. et al: PKC-beta controls I-kappa-B kinase lipid raft recruitment and activation in response to BCR signaling. Nature Immun. 3: 780-786, 2002.

    官网链接:https://www.signalchem.com/product_details.php?id=7840

    商品关键词:PKC beta II siRNA Set I,,P63-911-50,SignalChem

    简单描述:PKC beta II is a pool of three individual synthetic siRNA duplexes designed to knock-down human PKC beta II mRNA expression. Each siRNA is 19-25 bases in length.

    Description: PKC beta II is a pool of three individual synthetic siRNA duplexes designed to knock-down human PKC beta II mRNA expression. Each siRNA is 19-25 bases in length.

    Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.

    Format: Lyophilized powder

    Gene Aliases: PRKCB1; PKCB2; PRKCB1; PRKCB (X07109)


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    图标文献和实验
    相关实验
    • 【求助】PKC激活程度测定

      9921 Phospho-PKC Antibody Sampler Kit 9375 Phospho-PKCalpha/beta II (Thr638/641) Antibody W IP 9374 Phospho-PKCdelta (Thr505) Antibody W IHC 9376 Phospho-PKCdelta/theta (Ser643/676) Antibody W IHC 9377 Phospho-PKCtheta (Thr538

    • RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

      the anti-Flag-M2 beads. Response: This has been changed accordingly and the saved input is now in step 14 of Part-II. 5.For preparation of genomic DNA by Qiagen DNeasy kit, guanidine hydrochloride would be used at the lysis step. Can DNA (or RNA)-siRNA

    • siRNA protocols

      7 promoter oligo (5'-GGTAATACGACTCACTATAG-3')Heat at 95℃ for 3min.Snap cool on ice.ii. Small scale transcription reaction Set up two reactions. One with the sense template and one with the antisense template.40μl water5μl 10x T7 Buffer*2μl 25mM NTPs (1mM

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