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- SignalChem
- 加拿大
- K424-911C-05
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
5 nmol
存储温度：The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
存储溶液：The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
参考文献：1. Cloos, P. A. C.et.al: The putative oncogene GASC1 demethylates tri- and dimethylated lysine 9 on histone H3. Nature 442: 307-311, 2006.2. Yang, Z.-Q.et.a: Identification of a novel gene, GASC1, within an amplicon at 9p23-24 frequently detected in esophageal cancer cell lines. Cancer Res. 60: 4735-4739, 2000.
官网链接：https://www.signalchem.com/product_details.php?id=7647
商品关键词：KDM4C siRNA Set I,,K424-911C-05,SignalChem
简单描述：KDM4C is a pool of three individual synthetic siRNA duplexes designed to knock-down human KDM4C mRNA expression. Each siRNA is 19-25 bases in length.
Description: KDM4C is a pool of three individual synthetic siRNA duplexes designed to knock-down human KDM4C mRNA expression. Each siRNA is 19-25 bases in length.
Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Format: Lyophilized powder
Gene Aliases: KDM4C, GASC1, JHDM3C, JMJD2C
官网链接: www.annoron.com
办公电话: 010-56256916
官方售后: 400-965-8633
企业QQ: 3198592576、3212874516
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文献和实验mRNA 区域设计 siRNA。 2.选择 GC 含量低的 siRNA 研究人员发现 G/ C 含量 40–55％ 的 siRNA 活性比 G/ C 含量高于 55％ 的高。 3.纯化体外转录的 siRNA 确保所有用于转染的 siRNA 大小和纯度。为了实现最高级别的纯度，我们建议使用玻璃纤维过滤器结合和洗脱，或用凝胶纯化。所用的凝胶为 15- 20％ 的丙烯酰胺凝胶，以除去反应体系中多余的核苷酸、短低聚物、蛋白质和盐。注意：化学合成的 RNA 通常需要通过凝胶电泳进行纯化。 4.谨防RNA
., Jarvis, R., Pallotta, V., Byrom, M., and Ford, L. (2002) RNA interference in mammalian cell culture: design, execution, and analysis of the siRNA effect. Ambion TechNotes 9(1): 3-5. 4. Sui, G., Soohoo, C., Affar, E.B., Gay, F., Shi, Y., Forrester, W.C
interference in mammalian cell culture: design, execution, and analysis of the siRNA effect. Ambion TechNotes 9(1): 3-5. 4. Sui, G., Soohoo, C., Affar, E.B., Gay, F., Shi, Y., Forrester, W.C., and Shi, Y. (2002) A DNA vector-based RNAi technology
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