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IP-10 siRNA Set I

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  • ¥16908
  • SignalChem
  • 加拿大
  • 2025年07月16日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      安诺伦(北京)生物科技有限公司

    • 规格

      20 nmol

    研究领域:AKT/PKB Pathway, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway, JNK/SAPK Pathway, NfkB Pathway, p38 Pathway, PKA/PKC Pathway,

    存储温度:The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

    存储溶液:The siRNAs are supplied as a lyophilized powder and shipped at room temperature.

    参考文献:1. Angiolillo, A. L. et.al: Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. J. Exp. Med. 182: 155-162, 1995. 2. Dufour, J. H. et.al: IFN-gamma-inducible protein 10 (IP-10; CXCL10)-deficient mice reveal a role for IP-10 in effector T cell generation and trafficking. J. Immun. 168: 3195-3204, 2002.

    官网链接:https://www.signalchem.com/product_details.php?id=7616

    商品关键词:IP-10 siRNA Set I,,I838-911-20,SignalChem

    简单描述:IP-10 is a pool of three individual synthetic siRNA duplexes designed to knock-down human IP-10 mRNA expression. Each siRNA is 19-25 bases in length.

    Description: IP-10 is a pool of three individual synthetic siRNA duplexes designed to knock-down human IP-10 mRNA expression. Each siRNA is 19-25 bases in length.

    Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.

    Format: Lyophilized powder

    Gene Aliases: C7; IFI10; INP10; crg-2; mob-1; SCYB10; gIP-10; CXCL10


    官网链接: www.annoron.com
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    • SiRNA 实验十大秘诀

      于您的试验靶标 siRNA。) 转染 48 小时后,测量对照蛋白或 mRNA 水平相对于未转染细胞的减少量。太多的 siRNA能导致细胞毒性和死亡。赛默飞提供适用于各种靶标基因的阳性对照 siRNA。 9.使用阴性对照 siRNA 来区分非特异性效应 通过打乱最活跃的 siRNA 碱基序列排列,可以设计一个适当的阴性对照。一定要做好同源性搜索,以确保它与正在研究的生物体基因组缺乏同源性。 10. 使用标记的 siRNA 来进行试验方案优化 荧光标记的 siRNA 可用于分析 siRNA 的稳定性和转染

    • siRNA设计指南

      of variation in the length of nucleotide sequence being used as the stem of siRNA expression cassette. Several research groups including Ambion have used 19 nucleotides-long sequences as the stem of siRNA expression cassette (6-10). In contrast, other research

    • siRNA Design Guidelines

      . Several research groups including Ambion have used 19 nucleotides-long sequences as the stem of siRNA expression cassette (6-10). In contrast, other research groups have used siRNA stems ranging from 21 nucleotides-long (4-5) to 25-29 nucleotides-long (11

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