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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
安诺伦(北京)生物科技有限公司
- 规格:
20 nmol
存储温度:The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
存储溶液:The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
参考文献:1. Angiolillo, A. L. et.al: Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. J. Exp. Med. 182: 155-162, 1995. 2. Dufour, J. H. et.al: IFN-gamma-inducible protein 10 (IP-10; CXCL10)-deficient mice reveal a role for IP-10 in effector T cell generation and trafficking. J. Immun. 168: 3195-3204, 2002.
官网链接:https://www.signalchem.com/product_details.php?id=7616
商品关键词:IP-10 siRNA Set I,,I838-911-20,SignalChem
简单描述:IP-10 is a pool of three individual synthetic siRNA duplexes designed to knock-down human IP-10 mRNA expression. Each siRNA is 19-25 bases in length.
Description: IP-10 is a pool of three individual synthetic siRNA duplexes designed to knock-down human IP-10 mRNA expression. Each siRNA is 19-25 bases in length.
Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Format: Lyophilized powder
Gene Aliases: C7; IFI10; INP10; crg-2; mob-1; SCYB10; gIP-10; CXCL10
官网链接: www.annoron.com
办公电话: 010-56256916
官方售后: 400-965-8633
企业QQ: 3198592576、3212874516
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文献和实验于您的试验靶标 siRNA。) 转染 48 小时后,测量对照蛋白或 mRNA 水平相对于未转染细胞的减少量。太多的 siRNA能导致细胞毒性和死亡。赛默飞提供适用于各种靶标基因的阳性对照 siRNA。 9.使用阴性对照 siRNA 来区分非特异性效应 通过打乱最活跃的 siRNA 碱基序列排列,可以设计一个适当的阴性对照。一定要做好同源性搜索,以确保它与正在研究的生物体基因组缺乏同源性。 10. 使用标记的 siRNA 来进行试验方案优化 荧光标记的 siRNA 可用于分析 siRNA 的稳定性和转染
of variation in the length of nucleotide sequence being used as the stem of siRNA expression cassette. Several research groups including Ambion have used 19 nucleotides-long sequences as the stem of siRNA expression cassette (6-10). In contrast, other research
. Several research groups including Ambion have used 19 nucleotides-long sequences as the stem of siRNA expression cassette (6-10). In contrast, other research groups have used siRNA stems ranging from 21 nucleotides-long (4-5) to 25-29 nucleotides-long (11
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