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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-9478R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
SARP1
- 抗体名:
分泌细胞凋亡相关蛋白1抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human SFRP2/SARP1:201-295/295
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | SARP1 |
| 中文名称 | 分泌细胞凋亡相关蛋白1抗体 |
| 别 名 | Frizzled related protein 2; FRP 2; FRP-2; FRP2; SARP 1; SARP-1; SARP1; SDF 5; SDF5; Secreted apoptosis related protein 1; Secreted apoptosis-related protein 1; Secreted frizzled related protein 2; Secreted frizzled-related protein 2; sFRP 2; sFRP-2; sFRP2; Sfrp2 protein; SFRP2_HUMAN. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 心血管 信号转导 干细胞 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 33kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SFRP2/SARP1:201-295/295 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. Subcellular Location: Secreted. Tissue Specificity: Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. Similarity: Belongs to the secreted frizzled-related protein (sFRP) family. Contains 1 FZ (frizzled) domain. Contains 1 NTR domain. SWISS: Q96HF1 Gene ID: 6423 Database links: Entrez Gene: 6423 Human Entrez Gene: 20319 Mouse Entrez Gene: 310552 Rat Omim: 604157 Human SwissProt: Q96HF1 Human SwissProt: P97299 Mouse Unigene: 481022 Human Unigene: 19155 Mouse Unigene: 102416 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample: Hela (Human) Lysate at 40 ug Primary: Anti-SARP1 (bs-9478R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 36 kD |
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文献和实验——组成①蛋白质:普通蛋白质——生物发生:细胞溶质蛋白、细胞膜融合和转运相关蛋白、信号转导蛋白、热休克蛋白、四次跨膜超家族等;细胞特异性蛋白质——特定功能:免疫球蛋白超家族、共刺激分子、肿瘤抗原等。②胆固醇与脂类富含胆固醇;脂类:鞘磷脂、磷脂酰丝氨酸、神经节甘苷脂等③核酸携带的 mRNA 成分可以进入细胞浆中可以被翻译成蛋白质;exosomes 所转移的 microRNA 在进入靶细胞后可以靶向调节细胞中 mRNA 的水平。3. 外泌体的产生与生物学特性——生理功能细胞通讯功能:①免疫调节②凋亡③
细胞凋亡的相关蛋白分析 已发现有许多基因参与调控细胞凋亡,这些基因编码的产物可参与促进或抑制细胞凋亡的发生、发展。在此主要讨论其中几种主要的细胞凋亡调节基因蛋白的特征及其检测。 起初对凋亡调节基因的研究均以一种秀丽隐杆线虫(C.elegans)为模型。大量研究表明线虫细胞死亡基因(caenorhabditis elegans cell death gene, ced gene)与此线虫体细胞凋亡有关。其中,ced-3和ced-4基因所编码的蛋白可激活细胞凋亡
以维持自稳。 2)自噬过程很快,被诱导后8min即可观察到自噬体(autophagosome)形成,2h后自噬溶酶体(autolysosome)基本降解消失。这有利于细胞快速适应恶劣环境。 3)自噬的可诱导特性:表现在2个方面,第一是自噬相关蛋白的快速合成,这是准备阶段。第二是自噬体的快速大量形成,这是执行阶段。 4)批量降解:这是与蛋白酶体降解途径的显著区别。 5)“捕获”胞浆成分的非特异性:由于自噬的速度要快、量要大,因此特异
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