手机验证
上海联迈生物工程有限公司
大量
LM-9291R
多克隆
Rabbit
1年
MUL1
E3泛素连接酶MUL1抗体
Rabbit
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
KLH conjugated synthetic peptide derived from human MUL1/RNF218:1-100/352
IgG
Lyophilized or Liquid
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/test IF=1:50-200 (石蜡切片需做抗原修复)
1mg/ml
Store at -20 °C
100ul 200ul
英文名称 | MUL1 |
中文名称 | E3泛素连接酶MUL1抗体 |
别 名 | E3 ubiquitin-protein ligase MUL1; C1orf166; E3 ubiquitin ligase; E3 ubiquitin protein ligase MUL1; GIDE; Growth inhibition and death E3 ligase; MAPL; Mitochondrial anchored protein ligase; Mitochondrial ubiquitin ligase activator of NFKB 1; MUL1; MULAN; Putative NF kappa B activating protein 266; RING finger protein 218; RNF218; RP23-25C1.10-002; MUL1_HUMAN. |
规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 100ul 200ul |
研究领域 | 细胞生物 免疫学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/test IF=1:50-200 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 40kDa |
细胞定位 | 细胞浆 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MUL1/RNF218:1-100/352 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugatin. Function: Exhibits weak E3 ubiquitin-protein ligase activity, but preferentially acts as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Subunit: Homooligomer. Interacts with MAP3K7/TAK1. Interacts with UBC9. Interacts with and sumoylates DNM1L. Subcellular Location: Mitochondrion outer membrane; Multi-pass membrane protein. Peroxisome. Note: Transported in mitochondrion-derived vesicles from the mitochondrion to the peroxisome. Tissue Specificity: Widely expressed with highest levels in the heart, skeletal muscle, placenta, kidney and liver. Barely detectable in colon and thymus. Similarity: Contains 1 RING-type zinc finger. SWISS: Q969V5 Gene ID: 79594 Database links: Entrez Gene: 79594 Human Entrez Gene: 68350 Mouse Entrez Gene: 298576 Rat Omim: 612037 Human SwissProt: Q969V5 Human SwissProt: Q8VCM5 Mouse Unigene: 10101 Human Unigene: 103413 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-MUL1 (bs-9291R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40kD Observed band size: 40kD Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUL1) Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUL1) Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295g-cy3) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MUL1) Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295g-cy3) for 90 minutes, and DAPI for nuclei staining. Blank control: A549. Primary Antibody (green line): Rabbit Anti-MUL1/FITC Conjugated antibody (bs-9291R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed. |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。