- ¥780 - 2200
- LMAI Bio
- LM-9249R
- 进口/国产
- 2026年01月08日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Dog, Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-9249R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
RNF123
- 抗体名：
环指蛋白123抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Pig,
- 免疫原：
KLH conjugated synthetic peptide derived from human RNF123:1-100/1314
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
| 英文名称 | RNF123 |
| 中文名称 | 环指蛋白123抗体 |
| 别 名 | E3 ubiquitin protein ligase RNF123; Kip1 ubiquitination promoting complex protein 1; KPC 1; KPC1; Ring finger protein 123; RNF 123; Ubiquitin ligase; |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 149kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RNF123:1-100/1314 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in protein-protein interactions and protein-DNA interactions. RNF123 (RING finger protein 123), also known as KPC1 (Kip1 (p27) ubiquitination-promoting complex protein 1) or FP1477, contains one RING-type zinc finger domain and one SPRY domain. Localizing to the cytoplasm, RNF123 functions as the catalytic component of the KPC complex that acts as an E3 ubiquitin-protein ligase. Specifically, RNF123 is essential for the ubiquitination and subsequent degradation of p27 during the cell cycle G1 phase. Via its N-terminus, RNF123 is known to interact with GBDR1 (another component of the KPC) and p27 (a cyclin-dependent kinase inhibitor). Due to alternative splicing events, two isoforms exist for RNF123. Function: Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle. Subunit: Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2. Interacts with UBAC1 and CDKN1B via its N-terminal domain. Subcellular Location: Cytoplasmic Post-translational modifications: Ubiquitinated, leading to its degradation. Deubiquitinated by USP19, thereby stimulating CDKN1B ubiquitin-dependent degradation (By similarity). Similarity: Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger. SWISS: Q5XPI4 Gene ID: 63891 Database links: Entrez Gene: 63891 Human SwissProt: Q5XPI4 Human Unigene: 553723 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-RNF123 (bs-9249R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 149 kD Observed band size: 149 kD  Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-RNF123 Polyclonal Antibody, Unconjugated(bs-9249R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-RNF123 Polyclonal Antibody, Unconjugated(bs-9249R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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文献和实验123 药尊 是一条比较深吧。想完全没有第二条带，可能要买更好的抗体。 selleckchemcials 有两条带，说明你的抗体特异性不是很好~但是并不影响使用啊，那要看你是想做检验那个蛋白了~ 两种抗体同时做，肯定是一个出现一条带，另一个出现两条带，这样解释一下是会被审稿人认可的~ 如果想要更详细的有关ERK信号通路中抑制剂，抗体的信息，可以参照下面的网站： http://www
107 的 PBMC 细胞用 100μl 的 Buffer 稀释（Buffer CI：含 0.5% BSA 的 PBS 缓冲液，1 mM EDTA） · 加入多种生物素化抗体，室温下孵育 5 分钟。生物素化的抗体包含以下几种，每种抗体 0.5 μg：CD235a (erythrocytes), CD14 (monocytes), CD3 (T cells), CD15 (granulocytes), CD19 (B cells), CD36 (thrombocytes), CD123
性表达，或表达水平很低。在对新鲜分离的DC细胞的研究中，发现IL-3Ra在淋巴器官的DC细胞上有特异表达。从人类外周淋巴组织中分离的单个核细胞中主要是此类DC细胞。IL-3Ra（CD123）抗体可以用来进行免疫磁珠分选，从外周淋巴组织制备的单个核细胞样本中，将CD123+ DC细胞进行200倍富集。CD123抗体还可以用来做免疫组化分析，特异识别滤泡外T细胞富集区的CD123+ DC。在外周血中也有CD123+ DC细胞，与以前所说的CD11c- DC细胞和CD33dimCD14-CD16- DC
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