Human Anti-Hepatitis B virus s

Human Anti-Hepatitis B virus surface antibody ELISA Kit (Direct ELISA) - LS-F10240

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LS-F10240-1
1 plate
$715 USD
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10% off 5+ units
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Product Description

LS-F10240 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human Anti-Hepatitis B virus surface antibody in samples of Plasma and Serum. It is based upon a Direct assay principle.
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Specifications

Type
 
Direct ELISA (enzyme-linked immunosorbent assay) kit
Target
 
Anti-Hepatitis B virus surface antibody
Reactivity
 
Human
Specificity
 
This assay has high sensitivity and excellent specificity for detection of human HBsAb. No significant cross-reactivity or interference between human HBsAb and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human HBsAb and all the analogs, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Plasma, Serum
Sample Size
 
50 - 100 µl
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Precision
 
Intra-assay: CV%<10% Inter-assay: CV%<15%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Coated assay plate
• Negative Control
• Positive Control
• HRP-conjugate
• 2 x Wash Buffer (20 x concentrate)
• Substrate A
• Substrate B
• Stop Solution
• Adhesive Strip (For 96 wells)

Background
 
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HBsAg. Samples are pipetted into the wells with HBsAg conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human HBsAb bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio 100% Guarantee.
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