LS-F10266 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human Anti-HPV16 antibody (IgM) in samples of Plasma and Serum. It is based upon a Direct assay principle.
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Specifications
Type
Direct ELISA (enzyme-linked immunosorbent assay) kit
Target
Anti-HPV16 antibody (IgM)
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human HPV16 antibody (IgM). No significant cross-reactivity or interference between human HPV16 antibody (IgM) and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human HPV16 antibody (IgM) and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Precision
Intra-assay: CV%<15% Inter-assay: CV%<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
Coated assay plate
Negative Control
Positive Control
HRP-conjugate (100 x concentrate)
HRP-conjugate Diluent
2 x Sample Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip (For 96 wells)
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human HPV16 antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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