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Human Anti-Coxsackie virus I a

ntibody (IgG) ELISA Kit (Direct ELISA) - LS-F10383
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  • $800
  • LSBio
  • 详见官方网站
  • LS-F10383
  • 美国
  • 2025年07月12日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 样本

      血清/血浆/组织匀浆液

    • 库存

      1000

    • 适应物种

      Human

    • 应用

      科研使用

    • 检测方法

      ELISA

    • 检测范围

      详见官方网站

    • 供应商

      康朗生物

    • 规格

      96T

    Human Anti-Coxsackie virus I antibody (IgG) ELISA Kit (Direct ELISA) - LS-F10383
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    Catalog
    Size
    Price
    LS-F10383-1
    1 plate
    $800 USD
    Available Discounts
    10% off 5+ units


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    Popular Anti-Coxsackie virus I antibody (IgG) Elisa Kits

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    Human Anti-Coxsackie virus I antibody (IgG) ELISA Kit (Direct ELISA) - LS-F10381
    Type: Direct ELISA
    Format: 96-Well Microplate
    Reactivity: Human
    Detection: Colorimetric - 450nm (TMB)
    Sample Types: Serum

    Product Description

    LS-F10383 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human Anti-Coxsackie virus I antibody (IgG) in samples of Serum. It is based upon a Direct assay principle.
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    Specifications

    Type
     
    Direct ELISA (enzyme-linked immunosorbent assay) kit
    Target
     
    Anti-Coxsackie virus I antibody (IgG)
    Reactivity
     
    Human
    Specificity
     
    This assay has high sensitivity and excellent specificity for detection of human Coxsackie virus (Cox ? ) antibody (IgG). No significant cross-reactivity or interference between human Coxsackie virus (Cox ?) antibody (IgG) and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human Coxsackie virus (Cox ?) antibody (IgG) and all the analogs, therefore, cross reaction may still exist.
    Manual
     

    Intended Sample Types
     
    Serum
    Sample Size
     
    50 - 100 µl
    Format
     
    96-Well Microplate
    Detection
     
    Colorimetric - 450nm (TMB)
    Precision
     
    Intra-assay: CV%<15% Inter-assay: CV%<15%
    Storage
     
    Short term: 4°C; Long term: see manual.
    Quality Assurance
     
    Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
    Kit Components
     
    • Coated assay plate
    • Negative Control
    • Positive Control
    • Sample Diluent
    • HRP-conjugate
    • Wash Buffer (20 x concentrate)
    • Substrate A
    • Substrate B
    • Stop Solution
    • Adhesive Strip (For 96 wells)

    Background
     
    This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human Coxsackie virus (Cox ?) antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Restrictions
     
    For research use only.
    Guarantee
     
    This elisa kit carries the LSBio 100% Guarantee.
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    Direct ELISA Platform Overview
    Direct ELISA Platform Overview

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    相关实验
    • (共享)ELISA原理与实验方法

      , incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS

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      15. DPBS Without Calcium or Magnesium 16. TRIzol® LS Reagent 17. SuperScript® VILO™ cDNA Synthesis Kit 18. AccuPrime™ SuperMix I 19. Mouse Anti-Tra1-60 Antibody 20. Mouse Anti-Tra1-81 Antibody 21. Mouse Anti-SSEA

    • 【分享】ELISA原理

      活性,但具有IgG特有的抗原性。IgG可被胃蛋白酶分解为两个片段,一个Fab双体,称F(ab')2,能和两个相同的抗原结合;另一片段类似Fc,随后被分解成小分子多肽,无生物活性。IgM是由五个单体组成的五聚体,含10个重链和10个轻链,具有10个抗原结合价,由于空间位置的影响,只表现为五个抗原结合价。IgM分子量约为900000,IgG分子量约为150000。机体被微生物感染后,先产生IgM抗体,然后产生IgG抗体。经过一段时间,IgM抗体量逐渐减少而消失,而IgG抗体可长期存在,在疾病痊愈后可持续数年

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