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- 详细信息
- 文献和实验
- 技术资料
- 样本:
血清/血浆/组织匀浆液
- 库存:
1000
- 适应物种:
Human
- 应用:
科研使用
- 检测方法:
ELISA
- 检测范围:
详见官方网站
- 供应商:
康朗生物
- 规格:
96T
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Product Description
LS-F10325 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Human Anti-Chlamydia pneumoniae antibody (IgM) in samples of Serum. It is based upon a Direct assay principle.Top of Page
Specifications
TypeDirect ELISA (enzyme-linked immunosorbent assay) kit
Target
Anti-Chlamydia pneumoniae antibody (IgM)
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human chlamydia pneumoniae (Cpn) antibody (IgM). No significant cross-reactivity or interference between human chlamydia pneumoniae (Cpn) antibody (IgM) and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human chlamydia pneumoniae (Cpn) antibody (IgM) and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Serum
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Precision
Intra-assay: CV%<15% Inter-assay: CV%<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- Coated assay plate
- Negative Control
- Positive Control
- Sample Diluent
- HRP-conjugate
- Wash Buffer (20 x concentrate)
- Substrate A
- Substrate B
- Stop Solution
- Adhesive Strip (For 96 wells)
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human chlamydia pneumoniae (Cpn) antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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Direct ELISA Platform Overview
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文献和实验consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
a simple ELISA method to detect human anti-antibody response.
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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