- ￥3080
- CompTech
- A336
- 美国
- 2025年07月11日
11 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 规格：
1.0 ml/vial
存储温度： -70oC or below. Minimize freeze/thaw cycles.
来源：Normal human serum (shown by certified tests to be negativefor HBsAg and for antibodies to HCV, HIV-1 and HIV-II).
存储溶液：5 mM sodium phosphate, 145 mM NaCl, pH 7.2
参考文献：Dodds, A.W. and Sim, R.B. editors (1997) Complement. A Practical Approach (ISBN 019963539) Oxford University Press, Oxford. Morgan, B.P. ed. (2000) Complement Methods and Protocols. (ISBN 0-89603-654-5) Humana Press, Inc., Totowa, New Jersey.
官网链接：http://www.complementtech.com/product-description/Dpl-Sera/Factor%20D-Dpl.htm
商品关键词：Factor D-Dpl,A336,CompTech
简单描述：Factor D-Dpl is normal human serum depleted of factor D by immunoaffinity chromatography. The product is tested for the absence of factor D by functional assays for alternative pathway activity and by immunodiffusion against anti-factor D. Factor D-Dpl is certified to possess a functional alternative pathway for complement activation after reconstitution with 2 µg factor D/mL (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). It is tested for and certified to contain functional classical pathway indicating that all other complement components necessary for classical and alternative pathway activation are present except for factor D. Lectin pathway activity is not routinely tested or certified, but it would be expected to be active.
Concentration: >40 mg protein/mL (see Certificate of Analysis for actual conc.)
Form: Frozen liquid
Activity: >80% versus NHS standard after reconstitution with factor D
Purity: No factor D detectable by immunodiffusion
Preservative: None, 0.22 µm filtered
Physical Characteristics : Factor D-Dpl is supplied as a clear, straw-colored liquid containing all proteins of normal human serum except complement factor D.
Function: Factor D-Dpl serum is functionally deficient in alternative pathway activity, but it possesses a fully functional classical pathway. It is tested for classical pathway activity with assays using antibody-sensitized sheep erythrocytes (CompTech #B200) and for alternative pathway function using rabbit erythrocytes (CompTech #B300). The depleted serum is reconstituted with 1.4 µg/mL factor D (CompTech #A136) and retested to verify that a fully functional alternative pathway is restored. The Certificate of Analysis provided with each lot gives a description of the assays and specific titers for the depleted and reconstituted sera compared to normal human serum.
Assays: The unit of classical pathway activity is the CH50 and the unit of alternative pathway activity is the AP50. Similarly, the unit used to quantitate the activity of factor D is the DH50. A DH50 unit is the amount of functional factor D needed to lyse 50% of 1.5 x 107 rabbit erythrocytes (CompTech #B300)) when that amount of factor D (CompTech #A136) is incubated with the recommended volume of Factor D-Dpl in GVBo (CompTech #B103) containing MgEGTA (CompTech #B106) in a total volume of 100 µL for 30 min at 37oC. Various MgEGTA concentrations have been reported from 3 mM to 13 mM. The amount of factor D yielding one unit of activity (50% Er lysis) indicates the sensitivity of the assay for factor D which is typically less than 2 ng factor D with 30 µL Factor D-Dpl. See the Certificate of Analysis for lot specific values.
Precautions/Toxicity/Hazards: The source is human serum, therefore precautions appropriate for handling any blood-derived product must be used even though the source was shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-1 and HIV-II.
Hazard Code: B WGK Germany 3MSDS is available upon request.
官网链接: www.annoron.com
办公电话: 010-56256916
官方售后: 400-965-8633
企业QQ: 3198592576、3212874516
来源：Normal human serum (shown by certified tests to be negativefor HBsAg and for antibodies to HCV, HIV-1 and HIV-II).
存储溶液：5 mM sodium phosphate, 145 mM NaCl, pH 7.2
参考文献：Dodds, A.W. and Sim, R.B. editors (1997) Complement. A Practical Approach (ISBN 019963539) Oxford University Press, Oxford. Morgan, B.P. ed. (2000) Complement Methods and Protocols. (ISBN 0-89603-654-5) Humana Press, Inc., Totowa, New Jersey.
官网链接：http://www.complementtech.com/product-description/Dpl-Sera/Factor%20D-Dpl.htm
商品关键词：Factor D-Dpl,A336,CompTech
简单描述：Factor D-Dpl is normal human serum depleted of factor D by immunoaffinity chromatography. The product is tested for the absence of factor D by functional assays for alternative pathway activity and by immunodiffusion against anti-factor D. Factor D-Dpl is certified to possess a functional alternative pathway for complement activation after reconstitution with 2 µg factor D/mL (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). It is tested for and certified to contain functional classical pathway indicating that all other complement components necessary for classical and alternative pathway activation are present except for factor D. Lectin pathway activity is not routinely tested or certified, but it would be expected to be active.
Concentration: >40 mg protein/mL (see Certificate of Analysis for actual conc.)
Form: Frozen liquid
Activity: >80% versus NHS standard after reconstitution with factor D
Purity: No factor D detectable by immunodiffusion
Preservative: None, 0.22 µm filtered
Physical Characteristics : Factor D-Dpl is supplied as a clear, straw-colored liquid containing all proteins of normal human serum except complement factor D.
Function: Factor D-Dpl serum is functionally deficient in alternative pathway activity, but it possesses a fully functional classical pathway. It is tested for classical pathway activity with assays using antibody-sensitized sheep erythrocytes (CompTech #B200) and for alternative pathway function using rabbit erythrocytes (CompTech #B300). The depleted serum is reconstituted with 1.4 µg/mL factor D (CompTech #A136) and retested to verify that a fully functional alternative pathway is restored. The Certificate of Analysis provided with each lot gives a description of the assays and specific titers for the depleted and reconstituted sera compared to normal human serum.
Assays: The unit of classical pathway activity is the CH50 and the unit of alternative pathway activity is the AP50. Similarly, the unit used to quantitate the activity of factor D is the DH50. A DH50 unit is the amount of functional factor D needed to lyse 50% of 1.5 x 107 rabbit erythrocytes (CompTech #B300)) when that amount of factor D (CompTech #A136) is incubated with the recommended volume of Factor D-Dpl in GVBo (CompTech #B103) containing MgEGTA (CompTech #B106) in a total volume of 100 µL for 30 min at 37oC. Various MgEGTA concentrations have been reported from 3 mM to 13 mM. The amount of factor D yielding one unit of activity (50% Er lysis) indicates the sensitivity of the assay for factor D which is typically less than 2 ng factor D with 30 µL Factor D-Dpl. See the Certificate of Analysis for lot specific values.
Precautions/Toxicity/Hazards: The source is human serum, therefore precautions appropriate for handling any blood-derived product must be used even though the source was shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-1 and HIV-II.
Hazard Code: B WGK Germany 3MSDS is available upon request.
官网链接: www.annoron.com
办公电话: 010-56256916
官方售后: 400-965-8633
企业QQ: 3198592576、3212874516
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
指DNA双链的局部，由具有互补性单链 DNA与之结合所产生的环状结构。当 DNA复制开始，在原来的双链中仅一方被新合成的短 DNA单链被置换的情况下可以见到（为 Displacement loop之简称）。可通过人工使 DNA单链结合来制成此结构。由 RNA单链所产生的类似结构称为 R环。
of each component.2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly for the large
. The higher the pH dependence of the mobility, (dµ /dpH), the better the focusing. A high electrophoretic mobility close to the IpH will efficiently transfer diffused protein molecule back to IpH. This is essentially an intrinsic factor for the protein
技术资料暂无技术资料 索取技术资料
Factor D-Dpl
￥3080
