- ￥2547.79
- Biogems
- 美国
- 10122-25-100 ug
- 2025年07月12日
- FC, FA, IHC, IP
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
- 克隆性：
多克隆
- 供应商：
安诺伦（北京）生物科技有限公司
- 应用范围：
FC, FA, IHC, IP
- 规格：
100 ug
来源：Rat IgG2a, kappa
存储溶液：Phosphate-buffered aqueous solution, ph7.2.
参考文献：Ledbetter, J. A., & Herzenberg, L. A. (1979). Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*. Immunological reviews, 47(1), 63-90.Ledbetter, J. A., Rouse, R. V., Micklem, H. S., & Herzenberg, L. A. (1980). T cell subsets defined by expression of Lyt-1, 2, 3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. The Journal of experimental medicine,152(2), 280-295.Hathcock, K. (1991). T cell enrichment by cytotoxic elimination of B cells and accessory cells. Current protocols in immunology, 3-3.
特异性：Mouse
官网链接：http://www.bio-gems.com/flow-cytometry/safire-functional-antibodies/anti-mouse-cd8a-safire-purified.html
商品关键词：Anti-Mouse CD8a SAFIRE Purified,10122-25-100 ug,Biogems
简单描述：The 53-6.7 monoclonal antibody specifically reacts with Ly-2, the 38 kDa alpha chain, and with Lyt-2, the 34 kDa alpha’ chain, of the mouse CD8 antigen. The alpha’ chain is the truncated form of alpha chain, encoded by the same CD8a gene. In CD8a, the alpha and alpha’ chains form heterodimers with CD8b (the beta chains) or homodimers (alpha-alpha), which occur as receptors on the surface of the majority of thymocytes. A subpopulation of mature T lymphocytes expresses the CD8 alpha beta (alpha beta TCR T cells), and a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8a without CD8b.CD8 interacts with the mouse major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. Its function seems to be to attenuate the CD8-mediated signal for the stimulation of intrathymic T-cell maturation.The 53-6.7 antibody is useful for depleting CD8+ peripheral T lymphocytes. It cross reacts with the alpha- and alpha’-like polypeptides on some thymic and peripheral lymphocytes.
Clone: 53-6.7
Format: SAFIRE Purified
Cell Type: NK and NKT Cells, T Cells
官网链接: www.annoron.com
办公电话: 010-56256916
官方售后: 400-965-8633
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文献和实验-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
Immunohistochemistry using Anti-Ganglioside Antibodies
supernatant containing antiganglioside mAb overnight at 4°C. The time of incubation may range from 30 min. to overnight as determined by experience. 5. Wash with 1% BSA in PBS 4 times. 6. Incubate in FITC-conjugated goat anti-mouse IgM F(ab
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