简单描述:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFlt-3 Ligand. Anti-Human Flt-3 Ligand specific antibody was purified by affinity chromatography employing immobilized hFlt-3 Ligand matrix.
Sandwich ELISA: To detect hFlt-3 Ligand by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human Flt-3 Ligand (60-147BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFlt-3 Ligand.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml), a concentration of 4.0-6.0 ng/ml of this antibody is required.