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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-7088R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
CARD8
- 抗体名:
凋亡加强结构域蛋白8抗体
- 宿主:
Rabbit
- 适应物种:
Human
- 免疫原:
KLH conjugated synthetic peptide derived from human CARD8:221-320/431
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | CARD8 |
| 中文名称 | 凋亡加强结构域蛋白8抗体 |
| 别 名 | Apoptotic protein NDPP 1; Apoptotic protein NDPP1; CARD 8; CARD inhibitor of NF kappa B activating ligand; CARD inhibitor of NF kappaB activating ligand; CARD inhibitor of NF kappaB activating ligands; CARD-inhibitor of NF-kappa-B-activating ligand; CARD8; CARD8_HUMAN; CARDINAL; Caspase recruitment domain containing protein 8; Caspase recruitment domain family member 8; Caspase recruitment domain protein 8; Caspase recruitment domain-containing protein 8; DACAR; NDPP 1; NDPP1; TUCAN; Tumor up regulated CARD containing antagonist of CASP9; Tumor up regulated CARD containing antagonist of caspase 9; Tumor up-regulated CARD-containing antagonist of CASP9; Tumor upregulated CARD containing antagonist of CASP9. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 49kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CARD8:221-320/431 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. Function: Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. [SUBUNIT] May form homodimers. Interacts with NEMO and DRAL. Binds to caspase-1, CARD16/pseudo-ICE and CARD18/ICEBERG. Interacts with FNBP3 (By similarity). Interacts with NALP2 NACHT domain. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow. Similarity: Contains 1 CARD domain. SWISS: Q9Y2G2 Gene ID: 22900 Database links: Entrez Gene: 22900 Human Omim: 609051 Human SwissProt: Q9Y2G2 Human Unigene: 446146 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验独体—基于纤连蛋白类型Ⅲ结构域框架的抗体模拟 Akiko Koide and Shohei Koide
1. 介绍 蛋白质工程的一个主要目的是生产能够与特定靶分子结合的新的生物分子。这样的分子会应用于生物技术的许多领域,包括诊断、治疗和催化。虽然存在大量的天然蛋白,可供这些应用和开发,但用工程手段制造新的、有用的生物分子的办法仍是有吸引力的。 因为,它不受天然生物分子可用性的限制。抗体的基本构架中可变的链套呈现在基本不变的框架上,以及抗体工程的成功启发了一些研究组去发展“分子框架”[ 1,2] 。这些努力的主要目标一直是在保留呈递多重肽片段以与靶分子结合的能力的同时,克服抗体和抗体
三句话读懂一篇 CNS,酗酒背后的机制,环境-基因互作诱导肿瘤发生,Cas9 时钟...
Plus 2. Science: 发现 HIV 诱导细胞焦亡的炎症小体感受器 艾滋病作为一种危害性极大的传染病,会给人的生命健康带来巨大的威胁。 2021 年 2 月 4 日,美国圣路易斯华盛顿大学(WUSTL)的单梁教授团队在 Science 杂志发表论文 CARD8 is an inflammasome sensor for HIV-1 protease activity,首次报道了人体 CARD8 蛋白可以识别 HIV 蛋白水解酶的活性并形成炎症小体。当 HIV 感染原代 CD4+ T 细胞和巨噬细胞时,CARD8
Cell Death Differ:中大五院黄曦团队发现新冠病毒引起肺损伤的分子机制
共沉淀和线粒体胞质分离实验证实 SARS-CoV-2 M 蛋白和 BCL-2 家族蛋白促凋亡分子 BOK 相互作用,抑制 BOK 泛素化降解,并促进 BOK 发生线粒体转位(图 2)。BOK 主要通过 BH2 结构域与 M 蛋白结合。 图片来源:Cell death & differentiation 对小鼠进行气管暴露注射表达 M 蛋白慢病毒的实验表明,M 蛋白会导致小鼠肺上皮细胞凋亡和脱落,肺通透性增强和产生肺水肿,敲低 BOK 能减轻 M 蛋白导致小鼠肺部损伤(图 4)。 图片来源:Cell
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