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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
between chromatin elements without specifically identifying the protein components that mediate such interactions. Here we describe a detailed protocol for C ombined 3C -C hIP-C loning (6C) technology, which combines multiple techniques to identify the proteins
Quantitative Assessment of Reactive Metabolites
Quantitation of reactive intermediates from bioactivation of drug via covalent protein binding using radiolabeled drug is the gold standard for quantitation of reactive metabolite formation in the absence of synthetic standard
Purification of Endotoxin Free mAbs
Sulphate Precipitation Prepare saturated (NH4)2SO4, using Analar Grade reagent, 1kg per litre endotoxin free water, and autoclave in NaOH treated glass bottles. Store at 4 deg C. Mix supernatant above excess crystals before use to ensure
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