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发货周期:现货
博士德生物
cell culture supernates, serum and plasma(heparin).
Mouse
96T
货号 | EK0582 |
---|---|
产品名称 | Mouse Resistin ELISA Kit |
产品描述 | For quantitative detection of mouse Resistin in cell culture supernates, serum and plasma(heparin). |
免疫原 | E.coli, S21-S114 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <2pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | RETN |
基因全名 | resistin |
Uniprot ID | Q99P87 |
指标别名 | Adipose tissue specific secretory factor|Adipose tissue-specific secretory factor|ADSF|C/EBP epsilon regulated myeloid specific secreted cysteine rich protein|C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1|C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein|Cysteine rich secreted protein A12 alpha like 2|Cysteine rich secreted protein FIZZ3|Cysteine-rich secreted protein A12-alpha-like 2|Cysteine-rich secreted protein FIZZ3|FIZZ 3|FIZZ3|Found in inflammatory zone 3|HXCP 1|HXCP1|MGC126603|MGC126609|PRO1199|Resistin|Resistin delta2|RETN|RETN 1|RETN_HUMAN|RETN1|UNQ407|XCP 1|XCP1 |
背景资料 | Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin(for resistance to insulin), that may be the hormone potentially links obesity to diabetes.Resistin is overexpressed in mouse adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.The standard product used in this kit is mouse Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0582-CAP | 96-well plate precoated with anti- mouse Resistin antibody | 1 |
EK0582-ST | Lyophilized recombinant mouse Resistin standard | 10ng/tubex2 |
EK0582-DA | Biotinylated anti- mouse Resistin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 77 | 245 | 929 | 80 | 252 | 861 |
Standard deviation | 5.92 | 13.23 | 57.59 | 7.28 | 17.64 | 69.74 |
CV(%) | 7.7% | 5.4% | 6.2% | 9.1% | 7% | 8.1% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.006 | 0.122 | 0.245 | 0.441 | 0.743 | 1.101 | 1.748 | 2.241 |
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